|Table of Contents|

Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 03
Page:
397-401
Research Field:
Articles
Publishing date:

Info

Title:
Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*
Author(s):
WU Siyu ZHOU Zhiru YOU Qing ZHANG Wenfei XU Da LI Telun YAO Qi ZHANG Qichang WU Hongping & WANG Ruiping**
Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou 571158, China
Keywords:
water eutrophication mangrove phosphorus accumulating organisms screening Acinetobacter
CLC:
X172
PACS:
DOI:
10.3724/SP.J.1145.2015.11026
DocumentCode:

Abstract:
Dongzhaigang Mangrove Forest Nature Reserve, located in northeastern Hainan Island, is the major mangrove wetlands in China. The mangroves are evergreen trees that grow along the tropical or sub-tropical tidal shores or river estuaries. At high tide, they are totally submerged or only their tops are visible; when the tide recedes, they show themselves as dense thickets. The mangroves are periodically submerged by seawater, which have the same eco-environment of accumulation of large quantities of polyphosphate within their cells and the removal of phosphorus by Phosphorus Accumulating Organisms (PAOs). Thus, PAOs are selectively enriched in the bacterial community within the activated sludge of mangrove wetlands. In the present study, we screened 134 sludge samples isolated from the mangrove wetlands in Dongzhaigang and obtained 185 isolates of PAOs through the methods of BCIP blue-and white-colored screening, Albert staining and Nile Blue staining, which determined if the polyP or polyhydroxybutyrate (PHB) is on accumulation. The results showed a phosphorus removal rate of 42 PAOs as higher than 20%, and the maximum removal efficiency for phosphorus higher than 80%. Biolog phenotype microarrays and 16S rDNA sequences analysis were conducted to identify the isolates of PAOs. The result showed the populations of PAOs constituted by the bacterial genus of Acinetobacter sp., Bacillus sp., Stenotrophomonas sp., Pseudomonas sp. genera etc., with Acinetobacter being dominant in the entire communities. In our study, Dongzhaigang Mangrove Forest Nature Reserve is rich in PAO strains resources with good diversity. In this study, PAOs isolated from Dongzhaigang Mangrove Forest Nature Reserve are important in enhanced biological phosphorus removal and can be a candidate strain for preventing eutrophication in water bodies.

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Last Update: 2016-06-25