|Table of Contents|

The abnormal characteristics of oil and gas indicator microorganisms under the artificial environment of micro universe* (PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 03
Page:
415-422
Research Field:
Articles
Publishing date:

Info

Title:
The abnormal characteristics of oil and gas indicator microorganisms under the artificial environment of micro universe*
Author(s):
MEI Ze 1 XU Kewei2 TANG Yuping2 GU Lei2 YANG Fan2 YIN Bo2 & LIU He1**
1School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China 2Wuxi Research Institute of Petroleum Geology, Exploration and Production Research Institute, SINOPEC, Wuxi 214151, China
Keywords:
microbial prospecting microcosm 16S rRNA high-throughout sequencing gaseous hydrocarbon
CLC:
Q939.99 : TE357.9
PACS:
DOI:
10.3724/SP.J.1145.2015.09008
DocumentCode:

Abstract:
Microorganisms have been an important branch of surface oil and gas geochemical exploration. However, little is known about the function mechanism of hydrogen to microorganisms. This study adopted the incubation method and molecular biology to study the changes in the quantity and community structure of oil and gas hydrocarbon indicator microorganisms. The soil samples were taken from typical block and acclimated in micro-seepage (1 × 10-8) condition. The results showed that the quantity of oil and gas indicators all increased to different degrees. In groups acclimated by methane, the colonies of methane oxidizing bacteria increased by 76.5%, and the abundance of pmoA increased by 180.0%. In groups acclimated by butane, the colonies of butane oxidizing bacteria increased by 212.0%, and the abundance of bmoX increased by 285.0%. High-throughput sequencing further revealed that the oil and gas indicator microorganisms were low abundant population, with Methylococcaceae, Methylophilaceae, and Methylocystis (gas indicator bacteria) positively correlated with methane, Rhodospirillaceae, Pseudonocardia, and Methylobacter (oil indicator bacteria) positively correlated with butane, and other Nitrospira and Xanthomonas negatively correlated with hydrogen. This study intensified the understanding of function mechanisms about gaseous hydrocarbon to oil and gas microorganisms, to provide new ideas for oil and gas microorganisms exploration technology.

References

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Last Update: 2016-06-25