|Table of Contents|

Inflammatory effects of SO2 inhalation at low concentration in rat cortex(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2015 02
Page:
372-376
Research Field:
Articles
Publishing date:

Info

Title:
Inflammatory effects of SO2 inhalation at low concentration in rat cortex
Author(s):
YAO Gaoyi SANG Nan
1College of Environmental and Resource Sciences, Shanxi University, Taiyuan 030006, China 2Research Center of Environment and Health, Shanxi University, Taiyuan 030006, China
Keywords:
sulfur dioxide cerebral cortex gliacyte inflammatory cytokines extracellularly regulated kinase 1/2 cAMP response element-binding protein
CLC:
X174
PACS:
DOI:
10.3724/SP.J.1145.2014.09011
DocumentCode:

Abstract:
Sulfur dioxide (SO2), a ubiquitous air pollutant, is now being considered to be involved in neurotoxicity and many brain disorders via neuroinflammation. However, little is known about the impact of chronical SO2 inhalation on neuronal function. This paper aimed to investigate the impacts of chronical SO2 inhalation at low concentration on inflammation in rat cortex. Wistar rats were treated with different concentrations of SO2 (3.5 and 7 mg/m3) or filtered air 6 hours per day for 4 weeks, and the reactive astrocytes and microglia in rat cerebral cortex were determined by immunohistochemistry. The mRNA expression of inflammatory cytokines, tumor necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in rat cerebral cortex were investigated by qRT-PCR. The expression of neuroplasticity related proteins, phosphorylated cyclic adenosine monophosphate response-element binding protein (p-CREB) and phosphorylated extracellular signal-regulated kinase subtype 1/2 (p-ERK1/2) were investigated by immunoblot. The results showed that SO2 inhalation significantly stimulated activation of astroglial cells and inflammatory cytokine releases in rat cerebral cortex. The mRNA expression of TNFα, IL-1β, COX-2 and iNOS in rats treated with higher concentration of SO2 were 1.68, 1.58, 1.59 and 1.45 folds, respectively, higher than that of the control. Moreover, the expression of p-ERK1/2 and p-CREB in rats exposed to the higher concentration SO2 was 73% and 74% of that of the control. The results indicated that SO2 inhalation may cause neurological damage in rat cerebral cortex through neuroinflammation and abnormity of signal transduction in neuronal cytoplasm and gene transcription in the nucleus.

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Last Update: 2015-04-28