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Simultaneous production of energy from ammoxidation in microbial electrolysis cells(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 06
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Simultaneous production of energy from ammoxidation in microbial electrolysis cells
LI Jian ZHAN Guoqiang WANG Juan GAO Ping LI Daping
1School of Life Sciences, Sichuan University, Chengdu 610064, China 2Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China
microbial electrolysis cell ammoxidation denitrification hydrogen methane
X703 : TM911.45

In this study, a microbial electrolysis cell (MEC) was constructed with stainless steel tube as the cathode, carbon felt as the anode, nonwoven fabric as the separator between the two electrodes. Ammonia was the only electron donor, and mixed microbes inoculated in the cell. Through setting the different anode potentials, the products of ammonia oxidation under different ammonia concentrations were studied. The results showed that when the ammonium concentration was 200 mg/L and 400 mg/L, after 5 days’ operation under 0.2 V (vs Ag/AgCl) anode potential, the ammonia nitrogen removal rate reached 30% and 35% respectively. Additionally, 16.1 mL nitrogen and 1.18 mL methane were generated in the 200 mg/L system, while 17.18 mL and 1.46 mL for the 400 mg/L system. When the anode potential was adjusted to 0.6 V (vs Ag/AgCl), the ammonia nitrogen removal rate raised to 32.4% and 36.6% for the 200 mg/L and 400 mg/L system respectively. The production of nitrogen and methane were 16.48 mL and 1.3 mL for 200 mg/L system, while 17.42 mL and 1.52 mL for 400 mg/L system. During the operation period, no nitrate or nitrite nitrogen was detected. Cyclic voltammetry tests suggested that the anode had obvious redox peaks, and the location of the redox peaks would change by different anode potential. SEM indicated that the surface of the anode microorganisms had obvious fold shape. The high-throughput sequencing showed that Geobacter (24.11%) was the dominant population on the anode playing an important role in the ammonium oxidation process. The other functional microbes Synergistes (3.8%), Clostridium (3.8%) and Gordonia (1.85%) were also observed. The results of the experiment suggested that microorganisms are able to use ammonium as electron donor and produce hydrogen and methane in this MEC.


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Last Update: 2015-01-05