|Table of Contents|

 Transient expression of CHS-RNAi effectively influences the accumulation of anthocyanin in fruit of kiwifruit (Actinidia chinensis)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 05
Page:
929-933
Research Field:
Communications
Publishing date:

Info

Title:
 Transient expression of CHS-RNAi effectively influences the accumulation of anthocyanin in fruit of kiwifruit (Actinidia chinensis)
Author(s):
 SUN Jiaqi TANG Wei LIU Yongsheng
1Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China,
2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
Keywords:
 kiwifruit CHS anthocyanin transient expression qPCR
CLC:
S663.403
PACS:
DOI:
10.3724/SP.J.1145.2014.03021
DocumentCode:

Abstract:
Functional genomic studies on kiwifruit are very limited despite the fact that its genomic data is available. In the present study, we intended to establish an efficient system to investigate gene function by using RNA interference (RNAi) technique. A segment of cDNA putatively coding for chalcone synthase (CHS) derived from kiwifruit cv. Hongyang (Actinidia chinensis) was inserted into vectors pHB and pTCK303, to produce recombinant constructs 35S::CHS-RNAi and UBI::CHS-RNAi, respectively. The two recombinant plasmids were transformed into Agrobacterium tumefaciens EHA105 by liquid nitrogen. These two engineered A. tumefaciens strains were subsequently injected into the kiwifruits 106 days after full bloom (DAFB) when the red pigment of inner pericarp started to accumulate. About 0.7 mL engineered A. tumefaciens culture (about OD 0.8) was injected through fruit bottom into core tissue. As a result, 5 days after injection, as compared with blank control, a significant reduction of red pigment accumulation was visualized in inner pericarps injected with the engineered A. tumefaciens strains containing CHS-RNAi vectors, with remarkably decreased contents of anthocyanins detected at the same time. In addition, the CHS expression level was lower with the RNAi repression than that injected with empty vector. We anticipate that this rapid and transient expression system will be useful for studying the function of plant structural and regulatory genes in kiwifruit.

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Last Update: 2014-10-30