|Table of Contents|

 Characterization of major histocompatibility complex (MHC) class I loci exon 3 and exon 4 in three racerunner species (Squamata: Lacertidae: Eremias)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 05
Page:
877-886
Research Field:
Articles
Publishing date:

Info

Title:
 Characterization of major histocompatibility complex (MHC) class I loci exon 3 and exon 4 in three racerunner species (Squamata: Lacertidae: Eremias)
Author(s):
 YUAN Xiuyun LIU Jinlong ZENG Xiaomao GUO Xianguang
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
 MHC exon gene duplication trans-species polymorphism Eremias
CLC:
Q959.6+20.3
PACS:
DOI:
10.3724/SP.J.1145.2014.02003
DocumentCode:

Abstract:
The major histocompatibility complex (MHC) genes have been well characterized in mammals, birds, amphibians and fishes, but little is known about their organization in reptiles, despite the fact that reptiles occupy an important phylogenetic position for understanding the evolutionary history of vertebrates MHC genes. In this study we used polymerase chain reaction (PCR) and reverse transcription- polymerase chain reaction (RT-PCR) to amplify the genomic exon 3 and exon 4 fragments of MHC class I genes in three racerunner lizards, including viviparous Multiocellated Racerunner (Eremias multiocellata), viviparous Gobi Racerunner (Eremias przewalskii), and oviparous Ordos Racerunner (Eremias brenchleyi). We also presented the molecular characterization of class I sequences for racerunner lacertids. Gene duplication was detected in the three Eremias species, at least four MHC class I loci in Multiocellated Racerunners and Ordos Racerunners, and at least five in Gobi Racerunners. A putative class I pseudogene was inferred in Ordos Racerunner for a premature stop codon in a segment of so-called exon 3. The peptide binding region, β2-microglobulin interaction sites and a conserved cysteine residue were recognized in α2 domain encoded by exon 3. The β2-microglobulin interaction sites, CD8 molecule interaction sites and a conserved cysteine residue were recognized in α3 domain encoded by exon 4. Putative classical class I genes were expressed in the uterus of both oviparous and viviparous racerunners during pregnancy, along with some alleles unexpressed in uterine. In addition, phylogenetic reconstruction suggested the existence of trans-species polymorphism in MHC class I genes in Eremias. These findings provide clues for further investigations of co-evolution of viviparity and maternal immune system, as well as evolution of squamate MHC.

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