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Denitrification characteristics and community distribution of glycogen accumulating organism granules(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 04
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Denitrification characteristics and community distribution of glycogen accumulating organism granules
ZHANG Bin QIU Zhigang XUE Bin CHEN Zhiqiang GONG Taishi WANG Xue ZHI Weijia WANG Jingfeng
1Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China 2Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin 300050, China
glycogen-accumulating organisms (GAOs) denitrification granular sludge microbial community development biological nitrogen removal gaseous product tetrad-forming organisms (TFOs)
X703 : X172

This study aimed to investigate the denitrification ability and microbial ecological characteristic of glycogen-accumulating organisms (GAOs) during sludge granulation. A combined method of reactor process, batch test, microscale techniques and fluorescence in situ hybridization (FISH) was employed to evaluate the denitrification ability of GAOs, to reveal the microstructure of microbial communities and to characterize the distribution of GAOs and polyphosphate-accumulating organisms (PAOs) in different size granules. The results indicated that the adsorption rate of organic compound by sludge was over 92%. The SVI10 of granules stabilized at 30-50 mL g-1, a very low value compared to those measured for inoculating sludge (108.2 mL g-1). The nitrogen removal rate of nitrate and nitrite by GAOs granules reached 65% and 70% respectively, with N2 as the main gaseous product. The granules were tightly packed with a large amount of tetrad-forming organisms (TFOs), with filamentous bacteria serving as the supporting structures for the granules. FISH demonstrated that GAOs were distributed evenly throughout the granular space, whereas PAOs were severely inhibited and presented only in the edge of granules. A lab-scale sequencing batch reactor (SBR) could be successfully operated for cultivating GAOs granules in alternating anaerobic stirring-discharge-aerobic modes, with the GAOs granules showing excellent denitrifying capability. Therefore GAOs are the predominant member of the microbial population in granules.


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Last Update: 2014-08-26