|Table of Contents|

Antimicrobial activities and flavonoid contents of the extracts from four strains of duckweed(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 02
Research Field:
Publishing date:


Antimicrobial activities and flavonoid contents of the extracts from four strains of duckweed
XIE Tianyan HE Kaize ZHAO Hai PU Qiang FANG Yang
1 Chengdu Institute of Biology, Chinese Academy of Science, Chengdu, 610041, China 2 University of Chinese Academy of Science, Beijing, 100049, China
duckweed extracts antimicrobial activities total flavonoids

Duckweed (Lemnaceae) is a plant which grows in the sewage surface teeming with rich microbes, and it is proved that duckweed has a protective mechanism against microbes. In order to study the antibacterial mechanism of duckweeds and provide the basis for screening anti-bacterial substances and studying the infection mechanism of plant and animal pathogens or human pathogenic microorganisms, we first extracted using solvents with different polarities four strains of duckweed belonging to Lemna minor L. zh0018, Landoltia punctata zh0224, Spirodela polyrrhiza (L.) Schleid zh0003 and zh0225. The antimicrobial activities of their extracts were compared by Oxford Cup method and the minimum inhibitory concentration (MIC) measurement. Meanwhile, the contents and the composition of total flavonoids were determined by spectrophotometry combined with high performance liquid chromatography (HPLC) analysis. The results showed that the methanol extracts and 95% ethanol extracts of the duckweed had different degrees of antimicrobial activities against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Agrobacterium. The antimicrobial activity of the methanol extracts from S. polyrrhiza was the strongest (MIC: 6.25 mg/mL), followed by L. minor L. extracts; the ethyl acetate phase had the strongest activity (MIC: 0.78 mg/mL) followed by the n-butanol phase. The methanol extracts of L. minor L. had the highest level of total flavonoids content, 9.8% of the total extracts, followed by the 8.1% content of the methanol extracts of L. punctata. In addition, the ethyl acetate phase of S. polyrrhiza zh0225 methanol extracts had the highest content of total flavonoids (22.46%) followed by the 17.2% content of the n-butanol extracts. In HPLC measurement, the composition of flavonoids showed tremendous difference among the duckweed extracts, with the two strains of S. polyrrhiza having similar composition with different contents. The results suggested an enormous potential of S. polyrrhiza zh0225 as an excellent duckweed species for active substance extraction and pharmaceutical production.


1 朱晔荣, 马荣, 刘清岱, 戎清清, 杨琳, 王勇. 浮萍相关研究的几方面重要进展. 生物学通报, 2010, 45 (4): 4-6
2 Harvey RM, Fox JL. Nutrient removal using Lemna minor [J]. Water Pollut Control Fed, 1973, 45 (9): 1928-1938
3 Zirschky J, Reed SC. The use of duckweed for waste water treatment [J]. Water Pollut Control Fed, 1988, 60: 1253-1258
4 钱江华, 水生植物对水体污染物的清除及其应用[J]. 河北农业科学, 2008, 12 (10): 73-74 [Qian JH. Scavenging and application of aquatic plant to water pollutant [J]. J Hebei Agric Sci, 2008, 12 (10): 73-74]
5 罗铁成, 侯恩太, 陆峰, 巩江, 倪士峰, 崔延堂, 孙芳云. 浮萍药用研究概况[J]. 安徽农业科学, 2010, 38 (16): 8423-8424 [Luo TC, Hou ET, Lu F, Gong J, Ni SF, Cui YT, Sun FY. Overview of pharmacological research on Spirodela polyrhiza (L.) Schleid [J]. J Anhui Agricult Sci, 2010, 38 (16): 8423-8424]
6 Gao JP, Garrison AW, Hoehamer C, Mazur C, Wolfe NL. Uptake and phytotransformation of o, p’-DDT and p, p’-DDT by axenically cultivated aquatic plants [J]. J Agric Food Chem, 2000, 48 (12): 6121-6127
7 Gao J, Garrison AW, Hoehamer C, Mazur C, Wolfe NL. Uptake and phytotransformation of organophosphorus pesticides by axenically cultivated aquatic plants [J]. J Agric Food Chem, 2000, 48 (12): 6114-6120
8 Eid M, Kandil M, Moursy EB, Sayed G. Bioassays of duck weed vegetation extracts [J]. Intern J Trop Insect Sci, 1992, 13 (5): 741-748
9 王阳. 浮萍多糖的提取分离及其初步药效学研究[D]. 重庆: 重庆大学, 2004 [Wang Y. Research on extraction and isolation of polysaccharide from Herba Spirodelae seu Lemnae and its primary pharmacodynamics [D]. Chongqing: Chongqing University, 2004]
10 凌云, 何板作, 鲍燕燕, 郭秀芳, 郑俊华. 浮萍的化学成分研究[J]. 中草药, 1999, 30 (2): 88-89 [Ling Y, He BZ, Bao YY. Studies on the chemical constituents of common ducksmeat (Spirodela polyrrhiza ) [J]. Chin Trad Herb Drugs, 1999, 30 (2): 88-89]
11 何文妮, 叶敏, 王宝荣, 高慧媛, 果德安, 吴立军. 浮萍中黄酮类化学成分的分离与鉴定. 沈阳药科大学学报, 2010, 27 (11): 871-875
12 彭亮, 李知敏. 紫萍提取物对过氧化氢诱导内皮细氧化损伤的保护作用. 时珍国医国药, 2009, 20 (4): 996-998 [Peng L, Li ZM. The protective effect of Spirodela polyrrhiza (L.) Schleid extract on ECV-304 injury induced by hydrogen peroxide [J]. Lishizhen Med Mat Med Res, 2009, 20 (4): 996-998]
13 彭亮. 紫萍总黄酮提取纯化工艺及其抗癌抗氧化损伤的活性研究[D]. 重庆: 重庆大学, 2006 [Peng L. Study on extraction and purifieation technology of total flavonoids from Spirodela polyrrhiza (L.) Schleid and the bioactivity [D]. Chongqing: Chongqing University, 2004]
14 朱彤. 浮萍防止草鱼烂鳃病、肠炎病和赤皮病效果试验. 中兽医医药杂志[J], 2004 (2): 19-20 [Zhu T. The mechanism of duckweed in the prevention and cure of gill rot, enteritis and skin rot of grass carp [J]. J Trad Chin Veter Med, 2004 (2): 19-20]
15 黄晓冬. 赤楠叶不同极性提取物体外抗菌活性比较研究[J]. 武汉植物学研究. 2005, 23 (4): 355-357 [Huang XD. The comparative studies on antibacterial activities of extracts by different extracting solvents from the Syzygium buxifolium leaves [J]. J Wuhan Bot Res, 2005, 23 (4): 355-357]
16 元晓梅, 蒋明蔚, 胡正芝. 聚酰胺吸附-硝酸铝显色法测定山楂及山楂制品中的总黄酮含量[J]. 食品与发酵工业, 1996 (4): 27-32 [Yuan XM, Jiang MW, Hu ZZ. Colorimetric determination of Flavones in Hawthorn and its products [J]. Food Ferment Ind . 1996 (4): 27-32]
17 张书华, 张捷. 标准曲线法测定南山楂中总黄酮的含量[J]. 延安大学学报(医学科学版), 2012, 10 (1): 45-46
18 Qiao X, He WN, Xiang C, Han J, Wu LJ, Guo DA, Ye M. Qualitative and quantitative analyses of flavonoids in Spirodela polyrrhiza by high-performance liquid chromatography coupled with mass spectrometry [J]. Phytochem Anal, 2011, 22: 475-483
19 Zhang Y, Hu Yb, Yang BY, Ma F, Lu P, Li LM,Wan CS, Rayner S, Chen SY. Duckweed (Lemna minor) as a model plant system for the study of human microbial pathogenesis [J]. PloS ONE, 2010, 5 (10): e13527
20 黄晓冬. 4种龙眼核提取物的总黄酮含量、体外抗菌活性与抗氧化活性[J]. 食品科学, 2011, 32 (11): 43-47 [Huang XD. Total flavonoid contents, antibacterial activities and antioxidant activities of four extracts from Dimocarpus longan seeds [J]. Food Sci, 2011, 32 (11): 43-47]


Last Update: 2014-05-04