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Antimicrobial activities and flavonoid contents of the extracts from four strains of duckweed(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 02
Page:
238-244
Research Field:
Articles
Publishing date:

Info

Title:
Antimicrobial activities and flavonoid contents of the extracts from four strains of duckweed
Author(s):
XIE Tianyan HE Kaize ZHAO Hai PU Qiang FANG Yang
1 Chengdu Institute of Biology, Chinese Academy of Science, Chengdu, 610041, China 2 University of Chinese Academy of Science, Beijing, 100049, China
Keywords:
duckweed extracts antimicrobial activities total flavonoids
CLC:
Q949.71+7.3
PACS:
DOI:
10.3724/SP.J.1145.2014.00238
DocumentCode:

Abstract:
Duckweed (Lemnaceae) is a plant which grows in the sewage surface teeming with rich microbes, and it is proved that duckweed has a protective mechanism against microbes. In order to study the antibacterial mechanism of duckweeds and provide the basis for screening anti-bacterial substances and studying the infection mechanism of plant and animal pathogens or human pathogenic microorganisms, we first extracted using solvents with different polarities four strains of duckweed belonging to Lemna minor L. zh0018, Landoltia punctata zh0224, Spirodela polyrrhiza (L.) Schleid zh0003 and zh0225. The antimicrobial activities of their extracts were compared by Oxford Cup method and the minimum inhibitory concentration (MIC) measurement. Meanwhile, the contents and the composition of total flavonoids were determined by spectrophotometry combined with high performance liquid chromatography (HPLC) analysis. The results showed that the methanol extracts and 95% ethanol extracts of the duckweed had different degrees of antimicrobial activities against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Agrobacterium. The antimicrobial activity of the methanol extracts from S. polyrrhiza was the strongest (MIC: 6.25 mg/mL), followed by L. minor L. extracts; the ethyl acetate phase had the strongest activity (MIC: 0.78 mg/mL) followed by the n-butanol phase. The methanol extracts of L. minor L. had the highest level of total flavonoids content, 9.8% of the total extracts, followed by the 8.1% content of the methanol extracts of L. punctata. In addition, the ethyl acetate phase of S. polyrrhiza zh0225 methanol extracts had the highest content of total flavonoids (22.46%) followed by the 17.2% content of the n-butanol extracts. In HPLC measurement, the composition of flavonoids showed tremendous difference among the duckweed extracts, with the two strains of S. polyrrhiza having similar composition with different contents. The results suggested an enormous potential of S. polyrrhiza zh0225 as an excellent duckweed species for active substance extraction and pharmaceutical production.

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Last Update: 2014-05-04