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Molecular evolutionary characteristics of maize TASSELSEED2 and homologous genes in plant species(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 02
Page:
198-203
Research Field:
Articles
Publishing date:

Info

Title:
Molecular evolutionary characteristics of maize TASSELSEED2 and homologous genes in plant species
Author(s):
LI Shufen LIU Fang HU Lizong DENG Chuanliang GAO Wujun LU Longdou
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China
Keywords:
plant TS2 homologous genes sex determination molecular evolution
CLC:
Q941+.2
PACS:
DOI:
10.3724/SP.J.1145.2014.00198
DocumentCode:

Abstract:
Though TS2 is a sex determining gene initially found in maize, whether TS2 homologous genes in other monoecious and hermaphroditic plants are also related to the sex formation of flowers is not known. The evolutional characteristics of these genes are still not clear. In this study, 27 TS2 homologous genes from 21 sequenced plant genomes were identified by BLAST search using maize TS2 as query sequence, and a series of analysis were further performed, including their sequence characterization, phylogenetic relationships selective pressure, using bioinformatics and comparative genomics methods. Sequence analysis showed that TS2 genes from plants had three gene structure types, two typical functional domains and seven conserved motifs, with no specificity between monoecious and hermaphroditic plants. With SmoTS2 (Selaginellae Moellendorfii) protein as root of the phylogenetic tree, phylogenetic analysis indicated that except for PtrTS2b, SlaTS2, CsaTS2 and CpaTS2 which are all from monoecious plants, the remaining 22 plant TS2 proteins could be classified into four subgroups. It was worth noting that except for PtrTS2 from Populus trichocarpa, the other four TS2 genes from monoecious plants were far from each other, each belonging to a separate clade. Further selection analysis of the four subgroups detected a total of 24 positive selection sites (P > 0.05), indicating that all groups of TS2 genes were controlled by purifying selection or relaxed purifying selection. The above results indicated that plant TS2 genes underwent multiple gene duplication and amplification events, which deprived the TS2 genes of the function of sex determination or regulation. These results may provide useful clues for further investigating the plant TS2 gene function.

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Last Update: 2014-05-04