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Comparison of Two Triphenylmethane Dye (Malachite Green and Crystal Violet) in Fluorescence Detection(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2013 02
Page:
365-369
Research Field:
T & M
Publishing date:

Info

Title:
Comparison of Two Triphenylmethane Dye (Malachite Green and Crystal Violet) in Fluorescence Detection
Author(s):
ZHAO Yongyun DU Feng DONG Juan CHEN Haodong Zhou Li CHEN Rong TANG Zhuo
(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
Keywords:
malachite green crystal violet G-quadruplexe strand displacement amplification real-time fluorescence
CLC:
Q503 : Q78
PACS:
DOI:
10.3724/SP.J.1145.2013.00365
DocumentCode:

Abstract:
In order to select a triphenylmethane (TPM) dye to bind to G-quadruplex for developing a fluorescent quantitative reporting system, we compared the sensitivity and reproducibility of two TPM dyes, malachite green and crystal violet, binding to G-quadruplex. First, ?uorescence spectra of the two TPM dyes were compared in the presence of G-quadruplexes (hum21, short-hum21), single-strand and double-strands DNA. The results showed that both TPM dyes could discriminate the four DNAs with the sensitivity of malachite green higher than that of crystal violet. Then malachite green and crystal violet were applied to real-time fluorescence detection, which showed that malachite green had higher sensitivity and reproducibility than crystal violet. As a result, our proposed fluorescent quantitative reporting system is composed by malachite green and the G-quadruplex. Fig 4, Tab 1, Ref 21

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Last Update: 2013-05-03