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Isolation and Influencing Factors of Lactic Acid Bacteria with Microcystin-LR Degradation Ability(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2012 05
Page:
745-751
Research Field:
Articles
Publishing date:

Info

Title:
Isolation and Influencing Factors of Lactic Acid Bacteria with Microcystin-LR Degradation Ability
Author(s):
ZHANG Qian ZHANG Juan DU Guocheng CHEN Jian2
(1Key Laboratory of Industrial Biotechnology of Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi 214122, China)
(2State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
Keywords:
microcystin cyanobacteria screening and separation Lactobacillus casei biodegradation removal rate fermented food
CLC:
X172 : TQ920.1
PACS:
DOI:
10.3724/SP.J.1145.2012.00745
DocumentCode:

Abstract:
Microcystins, a kind of cyclic heptapeptide produced by cyanobacteria, are regarded as hepatotoxins and tumor promoters. In this case, removing cyanobacterial toxins plays an important role in the area of food security and environmental protection. In this study, 33 strains of lactic acid bacteria (LAB) isolated from many sorts of traditionally fermented pickles or sausages were identified, and their abilities to remove the cyanobacterial peptide toxin microcystin-LR (MC-LR) in aqueous solutions were assessed. Among them, the strain BBE10-212 showed the highest efficiency in toxin removal, and was classified phenotypically as a member of Lactobacillus casei on the basis of 16S rDNA sequencing. Furthermore, it was found that bacterial concentration, MC-LR concentration, cell viability and metabolic activity had a profound effect on the removal of MC-LR. In addition, the maximum removal of 52% was observed for BBE10-212 with 5% glucose supplementation compared with 19% removal in PBS alone. However, the existence of some inorganic nitrogen as nitrogen substitute of MC-LR and certain metal ions as key metabolic co-enzymes reduced the removal efficiency of the tested strain. The result of the study supplies a novel way to understand the removal mechanism of MC-LR. Moreover, it enlightens us to improve the removal efficiency of MC-LR with more strategies based on the metabolic control of strains. Fig 11, Tab 1, Ref 19

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