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Inhibitory Effect of Raddeanin A in Human Non-small Cell Lung Cancer H460 Cells(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2010 05
Page:
637-641
Research Field:
Articles
Publishing date:

Info

Title:
Inhibitory Effect of Raddeanin A in Human Non-small Cell Lung Cancer H460 Cells
Author(s):
GAO YarongAN JunxiaZHU QiyuLI FuMA ChaoyingWANG MingkuiTANG Yaxiong
(1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)
(2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
Keywords:
Raddeanin A H460 proliferation apoptosis cell cycle
CLC:
R965
PACS:
DOI:
10.3724/SP.J.1145.2010.00637
DocumentCode:

Abstract:
Raddeanin A, a triterpenoid saponin, was isolated from Anemone raddeana Regel and identified by solvent extraction and recrystallization. The effect of Raddeanin A on cell proliferation, apoptosis and cell cycle progression of human non-small cell lung cancer (NSCLC) H460 cancer cells and its mechanisms in this aspect were studied in this paper. The results demonstrated that Raddeanin A significantly suppressed proliferation of H460 cancer cells in a dose- and time-dependent manner which was associated with induced apoptosis and cell cycle G2-M arrest. Furthermore, Raddeanin A could inhibit Akt activation, down-regulate the expression of Bcl-2 protein, and activate the cleavage of PARP, leading to marked inhibitory effect of H460 cells. These findings suggest that Raddeanin A may be a potential usefulness for preventing and treating NSCLC. Fig 5, Ref 21

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Last Update: 2010-10-25