|Table of Contents|

Screening and identifying highly efficient crude oil-degrading and degrading enzymes-producing fungi(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2021 05
Page:
1289-1296
Research Field:
Articles
Publishing date:

Info

Title:
Screening and identifying highly efficient crude oil-degrading and degrading enzymes-producing fungi
Author(s):
WANG Qiuying1 ZHANG Guoqing2? CHEN Qingjun2 ZHANG Jiao1 LI Xiaofeng1 & XU Shiyi1
1 Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China 2 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China
Keywords:
crude oil degradation strain identification culture condition degrading enzyme
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2021.01025
DocumentCode:

Abstract:
Crude oil is called “industrial blood”, as it causes environmental pollution while it is extracted, processed, and transported. Therefore, 56 fungal strains collected from Inner Mongolia, Guangxi, and Beijing of China were screened and identified to determine which of their enzymes were highly efficient at degrading crude oil. The target strain JY-11 was obtained via a preliminary screening of crude oil tolerance followed by further screening with guaiacol, α-naphthol, and aniline blue plates. The strain was identified as Lentinus strigosus using morphological and molecular analyses. The optimum mycelial culture conditions were as follows: soluble starch and yeast extract as the carbon and nitrogen sources, respectively, in addition to a temperature of 36 ℃, C/N ratio of 20/1, and pH of 7.0. Strain JY-11 had a degradation rate of 70.88% ± 2.48% for crude oil after 10 d of shaking incubation at 30 ℃ and 150 r/min in a degradation medium with 0.5% crude oil. Subsequently, the JY-11 strain was shaking-cultured in a crude oil degradation medium supplemented with 0%-2% crude oil for 20 d at 30 ℃ and 150 r/min. The fermentation broth in each treatment showed laccase activity but no manganese peroxidase or lignin peroxidase activity. Native-PAGE results showed that crude oil induced laccase expression. The fermentation broth of the JY-11 strain also displayed high antioxidant properties. The antioxidant activity was 7.57 ± 0.01 mmol/L (FeSO4) at 48 h in the fermentation broth for the crude oil degradation medium in which crude oil had not been added. The 0.5% crude oil treatment showed the highest antioxidant activity at 11.83 ± 0.01 mmol/L (FeSO4). The present study identified a degrading fungus that could degrade crude oil highly efficiently via the secretion of extracellular enzymes. It has the potential to be applied in environmental remediation projects.

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Last Update: 2021-10-25