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Nutrient composition and microbial communities of bamboo at Wolong?National?Nature Reserve(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2021 05
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Nutrient composition and microbial communities of bamboo at Wolong?National?Nature Reserve
JIN Lei1# HE Yongguo2# YANG Xiaojun3 DENG Wenwen2 YANG Lin1 JIANG Chengyi1 LI Bei1 LI Caiwu2 ZHOU Yu2 ZENG Wen2 LI Ti2 HUANG Yan2 ZHANG Hemin2 ZHOU Shiqiang2 & ZOU Likou1?
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China 2 Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, China Conservation and Research Center for the Giant Panda (CCRCGP), Dujiangyan 611830, China 3 Wolong Special Administrative Region of Sichuan Province, Wolong 623004, China
Wolong giant panda bamboo nutrition composition microbiota

The giant panda is the flagship animal of the Wolong National Nature Reserve, which includes a high cover of many bamboo species in their diet. This study used chemical experiments and high-throughput ?sequencing to investigate the nutrition provided by and microbiota composition of the four main diet bamboo species preferred by giant pandas in Wolong.?The results showed that cellulose (46.18 ± 2.50% to 56.82 ± 2.2%), hemicellulose (11.74 ± 2.00% to 23.03 ± 2.74%), and lignin (15.29 ± 3.31% to 22.53 ± 6.0%) were the main components of bamboo, and no significant differences were found among hemicellulose and lignin between bamboo species (P > 0.05, Kruskal–Wallis test). The concentration of cellulose, flavone, and protein varied remarkably (P < 0.05, Kruskal–Wallis test) among different species of bamboo. The bacterial richness and fungal richness and diversity in different bamboo species fluctuated markedly (P < 0.05, Kruskal–Wallis test). Proteobacteria and Pseudomonas were the predominant bacterial phylum and genus at 81.3% and 26.2%, respectively. The abundances of Proteobacteria and Pseudomonas (86.2% and 33.9%, respectively) in Yushania brevipaniculata were significantly higher than those in the other groups (LDA > 4). Ascomycota was the most abundant fungal phylum in Phyllostachys bissetii (84.8%), Fargesia robusta (48.8%), and Bashania fangiana (48.8%), while Basidiomycota (37.7%) was the major fungal phylum in Y. brevipaniculata (60.4%). Cryptococcus (28.0%) was the predominant fungal genus in P. bissetii (56.4%), Y. brevipaniculata (57.4%), and B. fangiana (42.6%) while Rachicladosporium (11.0%) was the predominant fungal genus in F. robusta. In summary, our results indicate that different bamboo species provide significantly different levels of nutrition and have significantly different microbiota compositions. This research provides suggestions for the choice of bamboo species to be fed to captive giant pandas.


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Last Update: 2021-10-25