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Diversity and community structure of gut microbiomes of captive giant pandas in different regions(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2021 05
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Diversity and community structure of gut microbiomes of captive giant pandas in different regions
WANG Xin1 LI Caiwu2 JIN Lei1 ZHAO Lingling3 DENG Wenwen2 LI Ti2 XIONG Yuewu2 ZHANG Guiquan2 LI Guo2 HE Yongguo2 WU Daifu2 ZHANG Hemin2 HUANG Yan2? & ZOU Likou1?
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China 2 Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, China Conservation and Research Center for the Giant Panda (CCRCGP), Dujiangyan 611830, China 3 Nanjing Hongshan Forest Zoo, Nanjing 210028, China
giant panda captive high-throughput sequencing technology gut microbiome diversity

The gut microbiota of giant pandas are varied, with the age and living environment of the animals being important factors affecting the composition of their intestinal microbiota. This study investigated the regional differences therein by examining the structure and composition of bacteria from 74 giant pandas from different regions using high-throughput sequencing technology. The results showed that the most dominant phyla in the giant panda microbiota were Proteobacteria (61.95%) and Firmicutes (29.08%). At the genus level, the bacteria consisted mainly of Escherichia (24.92%), Pseudomonas (21.60%), Streptococcus (9.33%), Clostridium (9.58%), and Sphingobacterium (6.74%). When the giant pandas at the China Conservation and Research Center for the Giant Panda were transferred to other cities or regions, the core bacterial species–such as Escherichia, Pseudomonas, Streptococcus, and Clostridium remained present, although it appears that there were changes in their abundance and unique flora were also developed. There was no significant difference (P > 0.05) in the alpha diversity of sub-adult giant panda gut microbiomes; however, the gut microbiomes of sub-adult giant pandas in the Baoding and Guangzhou groups were significantly different (P < 0.05) based on beta diversity. The community diversity of the adult giant panda gut microbiomes in the Hetaoping and Shanghai groups was significantly different (P < 0.05) based on alpha diversity, while there was no significant difference among all adult giant pandas based on beta diversity (P >0.05). A similar gut bacterial community was found in giant pandas that ate the same kind of bamboo but otherwise were from widely different regions. Overall, geographical environments could probably affect the intestinal microbiota of captive giant pandas. Feeding these animals different kinds of bamboo has become one of the major factors causing a difference to develop in the intestinal microbial structure of captive giant pandas from different regions.


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Last Update: 2021-10-25