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Genome-wide identification and functional prediction of the RDRP gene family in Dimocarpus longan Lour.(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2021 05
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Genome-wide identification and functional prediction of the RDRP gene family in Dimocarpus longan Lour.
LIN Yiling XU Xiaoping ZHANG Chunyu CHEN Xiaohui ZHANG Zihao & LAI Zhongxiong?
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Dimocarpus longan Lour. RNA-dependent RNA polymerase gene (RDRP) gene family genome-wide identification function analysis

Bioinformatics analysis was used to clarify the biological functions of RNA-dependent RNA polymerase (RDRP) gene families in longan, and their chromosomal locations; molecular evolutionary tree; cis-acting promoter element; protein domain, secondary and tertiary structure, and interaction; alternative splicing types; and FPKM values were analyzed in somatic embryos. The results showed that the RDRP family in longan consisted of 10 members located on a total of 5 chromosomes, namely chromosomes 1, 8, 9, 11, and 15. By analyzing an evolutionary tree constructed using Dimocarpus longan Lour., Arabidopsis, and Oryza sativa, the genes were divided into four categories: DlRDR1, DlRDR2, DlRDR4, and DlRDR6. The RDRP family in longan contains an RDRP conserved domain while its protein family members have similar secondary and tertiary structures that can be associated with SGS3 and NRPD1A; this is particularly true of DCL proteins. Intron retention was the main splicing event at different stages of the somatic embryo and at different tissue sites. The analysis of gene expression patterns at different stages of somatic embryo development in the longan showed that RDRP was significantly upregulated in the non-embryonic stage. This study clarified how RDRP family members respond to plant hormones and participate in the growth and regulatory development of somatic embryos and different tissue parts in longan.


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