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Effect of antibiotics on degradation of 17β-estradiol by compound bacteria (PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2021 05
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Effect of antibiotics on degradation of 17β-estradiol by compound bacteria
HAO Peng1 ZHANG Xiqing1 GAO Yunhang1? LIU Jijun2 LU Wei3 HAN Yongsheng4 HAO Jiangang5 & CHENG Haijian6
1 College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China 2 College of Animal Science and Technology, China Agricultural University, Beijing 100083, China 3 Animal Disease Control Center of Chaoyang District, Changchun City, Changchun 130012, China 4 Qiqihar Experiment Station, National Beef Cattle Industrial Technology System, Qiqihar 161000, China 5 Wulagai Experiment Station, National Beef Cattle Industrial Technology System, Xilinhot 026000, China 6 Jinan Experiment Station, National Beef Cattle Industrial Technology System, Jinan 250000, China
17β-estradiol complex microbial system antibiotic degradation

To enrich 17β-estradiol (E2)-dominant degradation strains, this study aimed to construct optimal mixed strains from some functional bacteria. Six strains from the manure field were selected after the bacterial identification and Oxford cup bacteriostases experiment, and the E2 degradation rate of all six strains was above 25%. The optimal degradation conditions of the mixed strains were determined by analyzing the effects of temperature, pH, salinity, and substrate concentration on the degradation of E2. Due to the residual antibiotics in manure, six commonly used antibiotics were added to the medium to study the effect of different antibiotics on the degradation efficiency of E2 in mixed bacteria. The results showed that mixed strains A had a significant effect on the degradation of E2. The optimal conditions for degradation were a temperature of 30, initial pH7, substrate concentration of 20 mg/L. The degradation rate of E2 was up to 91.3% when cultured under the above conditions for 5 days. When the antibiotic concentration was 1 mg/L, sulfadiazine (SD) significantly promoted the degradation of E2 (P < 0.05), but oxytetracycline (OTC) and sulfamethoxazole (SMZ) inhibited E2 degradation (P < 0.05). Aureomycin (CTC), tylosin (TYL), and doxycycline (DC) had no significant influence on E2 degradation. When the antibiotic concentration was 10 mg/L, all antibiotics except SD significantly inhibited the degradation of E2 (P < 0.05), and OTC significantly inhibited the degradation, the rate of which was reduced by 9.6%. However, the degradation rate of mixed strain A to E2 was still over 80%. The mixed strains A constructed in this study can effectively improve the degradation efficiency of E2 and is less affected by antibiotics, which has a certain reference significance for the degradation of E2 by the microbial method.


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Last Update: 2021-10-25