|Table of Contents|

Excavation of genes involved in theacrine biosynthesis of Jiaochengkucha based on transcriptome(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2021 05
Page:
1382-1389
Research Field:
Articles
Publishing date:

Info

Title:
Excavation of genes involved in theacrine biosynthesis of Jiaochengkucha based on transcriptome
Author(s):
CHEN Xiaomin1 WANG Pengjie1 WANG Shuyan1 YANG Ruxing2 SUN Jun2 GUO Yongchun1 CHEN Xuejin1 ZHAO Feng3? & YE Naixing1?
1 College of Horticulture, Fujian Agriculture and Forestry University, Key Laboratory of Tea Science at Universities, Fuzhou 350002, China 2 Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350012, China 3 College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Keywords:
tea plant Jiaochengkucha theacrine caffeine transcriptome analysis
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2020.05041
DocumentCode:

Abstract:
To explore the molecular mechanism of the accumulation of theacrine in Fujian bitter tea Jiaochengkucha, the first tea germplasm resource containing theacrine in Fujian Province of China was selected as the object of this study, and Fudingdabaicha was used as the control cultivar. The alkaloid components were measured by HPLC, UFLC-Q-TOF-MS/MS, and their transcriptomes. The results showed that the alkaloid content in Jiaochengkucha was higher than that in Fudingdabaicha, and the highest content was theacrine (theacrine content: 25.0 ± 0.06 g/kg). Fudingdabaicha undetected the theacrine. Based on RNA-Seq, 48 unigenes were identified to be involved in the signaling pathway. KEGG pathway analysis showed that differentially expressed genes were annotated to four metabolic pathways: guanine degradation, adenine degradation, caffeine synthesis, and caffeine degradation. Further analysis showed that urate oxidase UOX (TEA012458) is downregulated, while tea caffeine synthase TCS (TEA030024) is upregulated in Jiaochengkucha. In short, the degradation pathways of guanine and adenine, and the pathways related to caffeine metabolism, were the important metabolic pathways that affect the synthesis of theacrine in Jiaochengkucha. Among them, urate oxidase UOX (TEA012458) and tea caffeine synthase TCS (TEA030024)?may be candidate genes regulating the synthesis of theacrine. These results provide a reference for the synthesis of theacrine in tea plants.

References

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Last Update: 2021-10-25