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Genome-wide identification and expression analysis of HAT gene family in longan(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2021 05
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Genome-wide identification and expression analysis of HAT gene family in longan
LI Xiaofei ZHANG Shuting SHEN Xu ZHANG Zihao CHEN Yukun LIN Yuling & LAI Zhongxiong?
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Dimocarpus longan Lour. acetylase gene genome-wide identification epigenetic regulation somatic embryogenesis expression pattern

Histone acetylase (HAT) is a histone lysine residue covalent modification component and plays a vital role in epigenetic regulation. To investigate the biological function and expression patterns of the HAT gene family in Dimocarpus longan Lour, we conducted genome-wide identification and bioinformatics analysis of the longan HAT gene family(DlHAT) based on a longan genome and transcriptome dataset. We detected the expression patterns of DlHAT in early somatic embryogenesis and different hormone treatments of longan by qRT-PCR.The results showed that the DlHAT gene family contains six members, namely DlHAC1, DlHAF1, DlHAM1, DlHAG1, DlHAG2, and DlHAG3. They were?distributed on six chromosomes of longan, all of which were hydrophilic proteins. Subcellular localization prediction showed that DlHAT was distributed in the nucleus and cytoplasm. Phylogenetic tree analysis showed that the DlHAT family could be divided into four categories: HAFs, HAGs, HACs, and HAMs, and the DlHAG family could be further divided into three subfamilies: GCN5, ELP3, and HAT1. Gene structure analysis revealed that the number of exons ranged from 8 to 30 in the DlHAT family, and most of their encoded proteins contained motif1, except for the HAG subfamily, HAFs, HACs, and HAMs subfamily encoded proteins containing motif6. The protein structure contained α-helix and random coils. The DlHAT promoter sequence contains many lights, hormones, stress, growth, and development-related cis-acting elements. qRT-PCR analyses of the early somatic embryos and different hormone treatments in longan revealed that DlHAF1, DlHAG1, and DlHAM1 were expressed at higher levels in the globular embryo (GE) stage, except for DlHAF, other DlHAT genes responded to salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), and gibberellin (GA3). The expression was upregulated after treatment with SA and GA3, and the expression levels were significantly upregulated at 8 h SA treatment and 4 h GA3 treatment. This study indicated that DlHAT is a conserved and species-specific characteristic during the evolution process and may be involved in long-term somatic embryonic development by responding to hormone regulation.


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Last Update: 2021-10-25