|Table of Contents|

Construction and application of immobilized laccase reactor(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2021 05
Page:
1297-1303
Research Field:
Articles
Publishing date:

Info

Title:
Construction and application of immobilized laccase reactor
Author(s):
LIN Zhiwei YE Xiuyun LIN Juan & YANG Jie?
Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou 350116, China
Keywords:
laccase enzyme immobilization bioreactor malachite green
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2020.04015
DocumentCode:

Abstract:
Laccases are valuable for a variety of industrial applications. Nonetheless, large-scale applications of laccases are limited by the economy and efficiency of the enzymes. Enzyme stability can be improved by immobilization. In the present study, the laccase from Cerrena sp. HYB07 was covalently immobilized on chitosan, and continuous decolorization of dyes was achieved with a lab-scale bioreactor, with guaiacol as the substrate; the immobilized enzyme activity was 43.5 U/g after optimization of immobilization conditions. Immobilized laccase displayed higher thermostability, with a half-life at 65 ℃, 5.3 times that of the free enzyme. The reactor was composed of an injection cell, reaction vessel, and collector, set to batch and cycle modes. The immobilized enzyme was used in an enzyme reactor to decolorize Remazol Brilliant Blue Reactive (RBBR) and malachite green (MG). After ten decolorization cycles, the activity of immobilized laccase and the decolorization rates of the dyes remained at 60% . Compared with the batch mode, the decolorization efficiency of the immobilized enzyme was higher in the circulating mode. Decolorization of MG by the immobilized enzyme was more thorough than that of the free enzyme. After laccase treatment, the microbial toxicity, phytotoxicity, and cytotoxicity of MG were significantly reduced. The stability and decolorization efficiency of laccase improved after immobilization. Furthermore, immobilized laccase has better operational stability in a bioreactor. The results of this study can prove the application value of laccase in dye decolorization and detoxification.

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Last Update: 2021-10-25