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Effect of the phenological stage of rice growth on soil-soluble inorganic nitrogen and bacterial communities in a yellow clayey soil under different fertilization patterns(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 06
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Effect of the phenological stage of rice growth on soil-soluble inorganic nitrogen and bacterial communities in a yellow clayey soil under different fertilization patterns
WANG Yi1 2 YANG Wenhao1 2 MAO Yanling1 2 ZHOU Biqing1 2 NIE San’an1 3 & XING Shihe1 2**
1 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Key Laboratory of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
fertilization pattern rice growth stage soil bacteria soluble inorganic nitrogen

The aim of this study was to reveal the dynamics of soil bacterial communities and their effects on the levels of soil-soluble inorganic nitrogen in paddy soil during the growth period of rice under different fertilization patterns. A yellow clayey paddy soil in the Fujian province with 33 years’ worth of different fertilization treatments including control (CK), chemical fertilizer (NPK), chemical fertilizer combined with cow manure (NPKM), and chemical fertilizer combined with rice straw (NPKS), was used in this study. The abundance and diversity of bacterial species, bacterial community structure, and levels of soil-soluble inorganic nitrogen in the paddy field during the seedling, tilling, booting, flowering and ripening stages were analyzed by real-time quantitative PCR and high-throughput sequencing. The results showed that the concentration of ammonium in the yellow clayey paddy soil ranged from 6.01 to 30.93 mg/kg and was the main source of soluble inorganic nitrogen in the soil. The 16S rRNA gene copy number ranged from 3.03 × 107 to 14.33 × 107 per g of dry soil, which showed that the abundance of soil bacteria varied greatly during the rice growth stages. The dominant bacterial communities in yellow clayey paddy soil were Proteobacteria, Chloroflexi, Actinobacteria, and Acidobacteria. Partial Least Squares Discriminant Analyses (PLS-DA) showed that the booting stage induces great variation in bacteria communities compared with other growth stages of rice. Also, inorganic fertilizers combined with organic fertilizers (NPKS and NPKM) showed great variation in comparison to different treatments. The permutational multivariate analysis of variance (PERMANOVA) results indicated that the rice growth stages accounted for 59.79% of the variation in the structure of the bacterial community while the treatment explained the 10.44% variation in the bacterial community structure. The soil ammonium concentration was positively correlated with the abundance of bacteria (P < 0.05). We concluded that the structure of the bacterial community in the soil was related to the concentration of ammonium nitrogen in paddy soil. Differences in the bacterial community structure were more likely to be influenced by the growth stages of rice rather than fertilization patterns.


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Last Update: 2019-12-25