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Effective butanol fermentation using corn starch and waste Pichia pastoris semi-solid mixture as the substrate

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 03
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Effective butanol fermentation using corn starch and waste Pichia pastoris semi-solid mixture as the substrate
XU Meng1 CHEN Cheng1 XIE Fang1 LUO Hongzhen2 DING Jian1 & SHI Zhongping1**
1 Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China 2 School of Life Science & Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China
acetone–butanol–ethanol (ABE) fermentation Clostridium acetobutylicum butanol waste solids recirculation & reduction starch sugar utilization yield

Developing a novel corn starch and waste Pichia pastoris semi-solid mixture substrate system for the acetone–butanol–ethanol (ABE) fermentation process can increase the low sugar utilization yield of traditional ABE fermentation, save on the usage and costs of raw materials, and reduce the amount of waste P. pastoris semi-solid produced and increase its reutilization. Semi-solid waste yeast was treated with NaOH to form solid-liquid suspensions, and then the pH of the suspensions was restored to 5-6 using H2SO4. The initial corn starch content was reduced from 15 to 8% to start the fermentation, and then the pretreated suspensions were added to a broth, where the ABE fermentation was then induced to enter the solventogenesis phase (after ~24 h) by supplementing the suspension with a suspension:broth mixture with a volume ratio of 1:4. Fermentation was implemented either in 100 mL anaerobic bottles or in a 7 L anaerobic fermenter. The optimal initial doses of corn starch, waste yeast suspension, and NaOH to use were identified as 8%, 80 g/L, and 6-10 g/L, respectively. With this mixture-substrate system, the butanol concentration increased and stabilized around 9.0-12.0 g/L, which was 80%-120% higher than that obtained with an 8 or 15% corn starch-based substrate, the total sugar utilization yield was greatly increased from below 50% to more than 90%, more than 57% of corn starch was saved, and a maximum 52% of the carbohydrates in the waste yeast semi-solid could be effectively reutilized or digested for gasification/liquefaction. A novel corn starch-waste yeast semi-solid mixture substrate-based ABE fermentation process was proposed based on the results of this study. This system simultaneously consumed a maximum 52% of the carbohydrates in the semi-solid waste for the formation of gaseous or liquid products to reduce the amount of waste semi-solid produced, increased the total sugar yield from 50% (in controls) to over 90%, stably maintained ABE/butanol concentrations of at least over 85% of the levels obtained using a 15% corn starch-based substrate, and saved over 57% of the expensive corn starch that would otherwise have to be used.


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Last Update: 2019-06-25