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Effect of controlled overexpression of XYL2 coding for xyltiol dehydrogenase by osmo-responsive promoters on xylose metabolism in Saccharomyces cerevisiae(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 06
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Effect of controlled overexpression of XYL2 coding for xyltiol dehydrogenase by osmo-responsive promoters on xylose metabolism in Saccharomyces cerevisiae
GU Hongyu1 2 ZHUGE Bin1 2 FANG Huiying1 2** ZONG Hong2 LU Xinyao2 CHEN Fanglin2 & ZHUGE Jian2
1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China 2Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University & Laboratory of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, China
osmo-responsive promoter xylitol dehydrogenase osmotic pressure Saccharomyces cerevisiae xylose

This research aimed to investigate the xylose metabolism in Saccharomyces cerevisiae with the xyltiol dehydrogenase gene XYL2 overexpressed at different levels by osmo-responsive promoters. Xylose-metabolizing strains were constructed by expressing the xylitol dehydrogenase gene XYL2 using osmo-responsive promoters PCgSTL3, PCgZWF and PCgGPD from Candida glycerinogenes in the multicopy plasmid pYX212. The activity of xylitol dehydrogenase and xylose-fermenting abilities of the recombination strains were measured. The recombinants with XYL2 gene expressed under osmo-responsive promoters were cultured with 0.4 mol/L NaCl as inducers. Increased activities of XDH were exhibited. The XDH activity of the recombinant SPG-xyl2 whose XYL2 was controlled by PCgGPD was approximately 2.8 times that of the recombinant whose XYL2 gene was expressed under PTPI. The strain SPG-xyl2 showed higher ethanol production (30.7%), ethanol yield (9.7%) and lower xylitol (53.3%) yield when cultivated in YPX with 0.4 mol/L NaCl. The results indicated that the XDH activity can be significantly improved by the overexpression of XYL2 gene using an osmo-responsive promoter PCgGPD. When the additional NaCl was added as osmo-inducer, the utilization of xylose was promoted with an increased?yield?of?ethanol as well as reduced accumulation of xylitol.


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Last Update: 2016-12-30