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The effect of ergosterol peroxide isolated from Amanita caesarea on the proliferation of ovarian cancer cells A2780S(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 06
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The effect of ergosterol peroxide isolated from Amanita caesarea on the proliferation of ovarian cancer cells A2780S
LUO Yu1 4 ZHOU Jing2 WU Xia2 GAO Ping1 & YUAN Xiaohong3**
1Laboratory of Medicinal Natural Products, College of Life Sciences, Sichuan University, Chengdu 610015, China 2Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China 3School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China 4Sichuan Aizhong Biological Medicine Science and Technology Co., Ltd, Chengdu 610041, China
ergosterol peroxide A2780S cell cell proliferation cell apoptosis cell cycle
Q949.329.810.6 : R965

This research aimed to study the proliferation of ovarian cancer cells A2780S induced by ergosterol peroxide isolated from Amanita caesarea. A2780S cells on the logarithmic growth phase were treated by ergosterol peroxide at the doses of 1.10, 2.19, 4.38, 8.75, 17.5 and 35 μmol/dL for 24, 48 and 72 h, and cell proliferation was determined by CCK-8 assay. After treatment at dose of 2.19, 4.38 and 8.75 μmol/dL for 24 h, the cell morphology was observed by inverted microscope and cell nuclear morphology was observed by DAPI assay. The early apoptosis rate of cells was determined by Annexin V/PI double-staining flow cytometry and the cell cycle was determined by PI cytometry. Ergosterol peroxide was found to inhibit the proliferation of A2780S. It increased the exfoliative and necrotic cells, and significantly increased the proportion of cells in S phase and early apoptosis of cells. The inhibition of A2780S cell proliferation by ergosterol peroxide may be through cell cycle blockage and cell apoptosis induction.


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Last Update: 2016-12-30