|Table of Contents|

Analysis on the specificity of methane-oxidizing bacteria media(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 06
Page:
1127-1133
Research Field:
Articles
Publishing date:

Info

Title:
Analysis on the specificity of methane-oxidizing bacteria media
Author(s):
YANG Fan1 2 SHEN Zhongming1 & MEI Ze3
1State Key Lab of Oil-gas Reservoirs Geology and Exploitation, Chengdu University of Technology, Chengdu 610059, China 2Wuxi Research Institute of Petroleum Geology, SINOPEC, Wuxi 214151, China 3School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China
Keywords:
culture medium plate count method methane oxidizing bacteria pmoA gene diversity analysis
CLC:
Q939.99 : P618.130.8
PACS:
DOI:
10.3724/SP.J.1145.2016.01023
DocumentCode:

Abstract:
This research tried to analyze and evaluate four methane oxidizing bacteria culture medium with different formula using plate count method, quantitative gene technology and T-RFLP analysis. We collected soil samples of oil-gas area and background area in Fuan Block. The experimental result showed that both the number of methane oxidizing bacteria and its associated pmoA gene abundance in bacterial liquid were higher in oil-gas field soil sample than in background soil sample under different media culture conditions. The difference in number of methane oxidizing bacteria between oil-gas field soil and background field soil was independent of the culture medium. ASI demonstrated that microbial anomalies could be easily identified with No.1 culture medium. T-RFLP analysis showed high biodiversity of methane oxidizing bacteria with all four culture media. Under the culture condition of No.1 culture medium, the abundance of methylococcus and methylocaldum, the two dominant oil-gas indicator microorganisms, was slightly higher than other communities, indicating the effectiveness of No.1 culture medium. This study confirms the reliability and accuracy of No.1 culture medium, and provides technical support for microbial prospecting of oil and gas.

References

1 王冰冰, 李治平. 油气微生物勘探技术的应用历史及前景分析[J]. 内蒙古石油化工, 2008, 34 (4): 24-28 [Wang BB, Li ZP. Application history and prospect analysis of microbial prospecting of oil and gas [J]. Inner Mongolia Petrochem Ind, 2008, 34 (4): 24-28] 2 Kolb S, Knief C, Stubner S. Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays [J]. Appl Environ Microbiol, 2003, 69: 2423-2429 3 Ehrlich HL. Geomicrobiology [M]. New York: Marcel Dekker, 2002 4 Tedesco SA. Surface Geochemistry in Petroleum Exploration [M]. New York: Chapman & Hall, 1995 5 Brown A. Evaluation of possible gas microseepage mechanisms [J]. AAPG Bull, 2000, 84: 1775-1789 6 梅博文, 袁志华, 王修垣. 油气微生物勘探法[J]. 中国石油勘探, 2002, 7 (3): 42 7 袁志华, 赵青, 王石头. 大庆卫星油田微生物勘探技术研究[J]. 石油学报, 2008, 29 (6): 827-831 [Yuan ZH, Zhao Q, Wang ST. Microbial prospecting technology for oil and gas in Satellite Oilfield of Daqing area [J]. Acta Petrol Sin, 2008, 29 (6): 827-831] 8 袁志华, 付晓宁. 鄂尔多斯盆地太昌-和盛区块油气微生物勘探研究[J]. 内蒙古石油化工, 2008, 34: 1006-7981 [Yuan ZH, Fu XN. Microbial prospecting of oil and gas (MPOG) in Taichang-hesheng Area of Ordos Basin [J]. Inner Mongolia Petrochem Ind, 2008, 34: 1006-7981] 9 王国建, 邓平, 夏响华. 微生物方法在油气勘探中的试验研究——以松辽盆地十屋断陷为例[J]. 天然气工业, 2006, 26 (4): 8-10 [Wang GJ, Deng P, Xia XH. Experiment study of microbiological petroleum exploration: Taking shiwu fault depression of the Songliao basin as an example [J]. Nat Gas Ind, 2006, 26 (4): 8-10] 10 张春林, 庞雄奇, 梅海. 微生物油气勘探技术在岩性气藏勘探中的应用-以柴达木盆地三湖坳陷为例[J]. 石油勘探与开发, 2010, 37 (3): 310-315 [Zhang CL, Pang XQ, Mei H. Application of microbial oil surveying to exploration of lithologic gas reservoirs: a case from the Sanhu Depression, Qaidam Basin, NW China [J]. Petrol Expl Dev, 2010, 37 (3): 310-315] 11 张春林, 庞雄奇, 梅海. 烃类微渗漏与宏渗漏的识别及镇巴长岭-龙王沟地区勘探实践[J]. 天然气地球科学, 2009, 20: 794-800 [Zhang CL, Pang XQ, Mei H. Identification of microseepage from macroseepage and exploration practice in Changling-Longwanggou Area of Zhenba Block [J]. Nat Gas Geosci, 2009, 20: 794-800] 12 邵明瑞, 许科伟, 汤玉平. 三种油气指示菌定量PCR方法的建立及其在油气田土壤中的初步应用[J]. 生物技术通报, 2013, 23 (4): 172-178 [Shao MR, Xu KW, Tang YP. The quantitative PCR technology for three oil and gas indicating bacteria and its preliminary application in oil and gas field soils [J]. Biotechnol Bull, 2013, 23 (4): 172-178] 13 张莹, 李宝珍, 杨金水.油气田土壤样品中可培养丁烷氧化菌多样性研究[J]. 环境科学, 2012, 33 (1): 229-304 [Zhang Y, Li BZ, Yang JS. Diversity of culturable butane-oxidizing bacteria in oil and gas field soil [J]. Chin J Environ Sci, 2012, 33 (1): 229-304] 14 闵航, 陈中云, 陈美慈. 水稻田土壤甲烷氧化活性及其环境影响因子的研究[J]. 土壤学报, 2002, 39 (5): 686-692 [Ming H, Chen ZY, Chen MC. Effect of environmental factors on methane-oxidizing acticity in paddy soil [J]. Acta Pedol Sin, 2002, 39 (5): 686-691] 15 Bender M, Conrad R. Effect of CH4 concentrations and soil conditions on the induction of CH4 oxidation activity [J]. Soil Biol Biochem, 1995, 27 (12): 1517-1527 16 Hutsch BW. Methane oxidation in arable soil as inhibited by ammonium, nitrite and manure with respect to soil pH [J]. Biol Fert Soils, 1998, 28 (1): 27-35 17 King GM, Schnell S. Effect of increasing atmospheric methane concentration on ammoninum inhibition of soil methane consumption [J]. Nature, 1994, 370 (6487): 282-284 18 陈中云, 闵航, 吴伟祥. 不同离子对水稻田土壤甲烷氧化活性影响的研究[J]. 植物营养与肥料学报, 2002, 8 (2): 219-223 [Chen ZY, Ming H, Wu WX. Effect of mineral nutrition ions on the activity of methane oxidation in paddy soil [J]. Plant Nutr Fertil Sci, 2002, 8 (2): 219-223] 19 闵航, 陈中云, 吴伟祥等. 碳、氮物质对水稻田土壤甲烷氧化活性影响的研究[J]. 环境科学学报, 2002, 22 (1): 70-75 [Ming H, Chen YZ, Wu WX, Chen MC. Effect of carbon and nitrogen sources on the activity of methane oxidization in a paddy rice soil [J]. Acta Sci Circumst, 2002, 22 (1): 70-75] 20 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社, 1999 21 魏文平, 邓辉, 李国学. 一株Ⅱ型甲烷氧化菌的筛选及培养条件[J]. 应用与环境生物学报, 2015, 21 (3): 455-463 [Wei WP, Deng H, Li GX. Screening and culture condition of a type II methanotroph [J]. Chin J Appl Environ Biol, 2015, 21 (3): 455-463] 22 余海霞. 利用微生物技术治理煤矿瓦斯的研究[D]. 杭州: 浙江大学, 2007 [Yu HX. Studies on bio-oxidizing methane in coal mines with microbial technology [D]. Hangzhou: Zhejiang University, 2007] 23 马强, 陶秀祥, 侯彤. 油田土壤中甲烷氧化菌的筛选及鉴定[J]. 煤炭工程, 2008 (7): 84-85 24 杨帆, 汤玉平, 许科伟. 海安凹陷富安油气区微生物异常分布研究[J]. 西安石油大学学报, 2014, 29 (6): 83-87 [Yang F, Tang YP, Xu KW. Study of microbial anomaly distribution in fuan oil-gas area, Haian Sag [J]. J Xi’an Shiyou Univ, 2014, 29 (6): 83-87] 25 Peng J, Lu Z, Rui J. Dynamics of the methanogenic archaeal commuity during plant residue decomposition in an anoxic rice field soil [J]. Appl Environ Microbiol, 2008, 74 (9): 2894-2901 26 Engebretson JJ, Moyer CL. Fidelity of select restriction endonucleases in determining microbial diversity by terminal-restriction fragment length polymorphism. Appl Environ Microbiol, 2003,,69 (8): 4823-4829 27 Denaro R, Auria G, Marco G. Assessing terminal restriction fragment length polymorphism suitability for the description of bacterial community structure and dynamics in hydrocarbon-polluted marine environment. Environ Microbiol, 2005, 7 (1): 78-87 28 杨旭, 许科伟, 刘和. 油气藏上方土壤中甲烷氧化菌群落结构分析——以沾化凹陷某油气田为例[J]. 应用与环境生物学报, 2013, 19 (3): 478-483. [Yang X, Xu KW, Liu H. Analysis of methane-oxidizing bacteria community structure in the soil above oil [J]. Chin J Appl Environ Biol, 2013, 19 (3): 478-483]

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Last Update: 2016-12-30