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Enrichment and algicidal factors of algicidal bacterial community(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 06
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Enrichment and algicidal factors of algicidal bacterial community
XIONG Qingshan1 PAN Qiuling1 WANG Qin1 ZHOU Zeyang1 2 & WANG Linling1**
1 College of Life Science, Chongqing Normal University, Chongqing 401331, China; 2State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
bacterial community efficient algicidal solution algicidal activity Microcystis aeruginosa

Eutrophication and harmful algae blooms have attracted more and more concern in China due to their adverse effects on the environment. It is imperative to seek effective methods to prevent algae blooms. Algae-lysing microbes has broad application prospect. The efficient algicidal solution was enriched by co-cultured algae bloom water and Microcystis aeruginosa repeatedly. The efficient algicidal solution showed strong and stable algicidal ability. The main characteristics of algae lysing active substance were analyzed by factors including centrifugation, membrane filtration, temperature and antibiotics. The result showed that algicidal substance was precipitated after centrifuging at 3 000 r/min for 5 min; the supernatant lost algicidal ability after centrifuging at 7 000 r/min for 5 min. The membrane filter treatment of efficient algicidal solution found the algicidal substance not able to pass 2 μm membrance. The algicidal activity was significantly lower after bathing in 45°C water for 10 min, and was completely lost after bathing in 48°C water for 10 min. Antibiotic treatment of efficient algicidal solution showed the algicidal ability was not influenced by adding cycloheximide, slightly impacted by chloramphenicol, and lost after tetracycline treatment, suggesting that the algicidal substance was not fungus, and that the effect of gram-positive bacteria were greater than gram-negative ones. Single colonies isolated by LB medium had no significant algicidal activity. The results indicated that algicidal substance of the efficient algicidal solution is bacterial community composed of a variety of bacteria.


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