|Table of Contents|

Construction of a multifunctional pesticide-degrading strain using Pseudomonas putida KT2440 as the host(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 06
Page:
1145-1149
Research Field:
Articles
Publishing date:

Info

Title:
Construction of a multifunctional pesticide-degrading strain using Pseudomonas putida KT2440 as the host
Author(s):
YI Zhongquan1 2 WANG Rui1 ZHOU Chaoyang1 CHEN Xueting1 HONG Qing1 HE Jian1 YAN Xin1** & LI Shunpeng1
1College of Life Science, Nanjing Agricultural University, Nanjing 210095, China 2Department of Central Laboratory, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng 224000, China
Keywords:
Homologous recombination microbial degradation of pesticides contamination Pseudomonas putida strain KT2440 genetically engineered microorganism
CLC:
X172
PACS:
DOI:
10.3724/SP.J.1145.2016.01006
DocumentCode:

Abstract:
Microbial in situ remediation is considered as a promising method to eliminate the pollution of pesticides. Generally, pesticide-degrading microorganisms are isolated from pesticide-contaminated sites and are then employed to remediate the pollution of pesticides. The shortcomings of these strains are narrow substrate spectrum, unstable biodegrading ability and unknown safety. Pseudomonas putida strain KT2440 is isolated from rhizosphere soil and is generally recognized as safe. This study aimed to construct a multifunctional pesticide-degrading microorganism using P. putida strain KT2440 as the host, with a hope of making a promising candidate for in situ bioremediation. Pesticide-degrading enzyme encoding genes pytH, mpd, chd and ampA were integrated into the chromosome of P. putida strain KT2440 via homologous recombination. The recombinant strain KT-pmca could degrade four pesticides including pyrethroids, organophosphorus, chlorothalonil and amide. Among them, the degradation rate of chlorothalonil was more than 95% and the degradation rate of chlorpyrifos, fenpropathrin and stam exceeded 80%. Furthermore, pesticide-degrading phenotype of P. putida strain KT-pmca was quite stable. This study showed that the recombinant strain KT-pmca could provide a promising strain for the in situ remediation of pesticides contamination.

References

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Last Update: 2016-12-30