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Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

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Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments
LI Xiaoliang1 2 ZHANG Liang2 ZHANG Peiyu1 & LIN Xuezheng2**
1College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China 2First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China
Arctic Ocean sediments low temperature nitrobacteria phylogenetic analysis sewage treatment

In cold areas, wastewater biotreatment techniques have some shortcomings such as low efficiency and difficulties in achieving emission standards because of the long cold winter and the low temperature of wastewater. The special environment and climate in polar regions has created a unique microbial ecosystem and species resources. On the basis of morphological characters, a total of 81 cold-adapted nitrobacteria were isolated from 12 Arctic Ocean sediments with four kinds of screening media. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that these isolates belonged to 4 phyla, 4 classes, 8 orders, 16 families, 22 genera, and 30 species. One isolate with an 16S rRNA gene sequence similarity less than 97% with the closest type strain might be a novel species. The growth temperature and NaCl tolerance range of most of the representative strains was 0–30 ℃ and 0–90‰, respectively. These isolates could produce many extracellular enzymes such as protease, urease, and β-galactosidase, and they could grow by utilizing many kinds of carbon sources, including glucose, mannitol, and maltose. This study can offer new microbial resources for improving sewage treatment at low temperature.


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Last Update: 2017-04-25