|Table of Contents|

Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017年第2期
Page:
294-300
Research Field:
Articles
Publishing date:
2017-04-25

Info

Title:
Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments
Author(s):
LI Xiaoliang1 2 ZHANG Liang2 ZHANG Peiyu1 & LIN Xuezheng2**
1College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China 2First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China
Keywords:
Arctic Ocean sediments low temperature nitrobacteria phylogenetic analysis sewage treatment
CLC:
X172
PACS:
DOI:
10.3724/SP.J.1145.2016.04004
DocumentCode:

Abstract:
In cold areas, wastewater biotreatment techniques have some shortcomings such as low efficiency and difficulties in achieving emission standards because of the long cold winter and the low temperature of wastewater. The special environment and climate in polar regions has created a unique microbial ecosystem and species resources. On the basis of morphological characters, a total of 81 cold-adapted nitrobacteria were isolated from 12 Arctic Ocean sediments with four kinds of screening media. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that these isolates belonged to 4 phyla, 4 classes, 8 orders, 16 families, 22 genera, and 30 species. One isolate with an 16S rRNA gene sequence similarity less than 97% with the closest type strain might be a novel species. The growth temperature and NaCl tolerance range of most of the representative strains was 0–30 ℃ and 0–90‰, respectively. These isolates could produce many extracellular enzymes such as protease, urease, and β-galactosidase, and they could grow by utilizing many kinds of carbon sources, including glucose, mannitol, and maltose. This study can offer new microbial resources for improving sewage treatment at low temperature.

References

1 李亚选, 负英伟, 焦瑞虎, 张晓玲, 姜安玺. 低温耐冷菌在寒冷地区生活污水处理中的应用[J]. 应用科技, 2007, 34 (4): 63-66 [Li YX, Fu YW, Jiao RH, Zhang XL, Jiang AX. Study on the psychrotrophilic bacteria for treatment of cryogenic domestic sewage [J]. Appl Sci Technol, 2007, 34 (4): 63-66]
2 李亚选, 张晓玲, 姜安玺, 孟雪征,韩晓云. 低温菌去除污染物的研究现状[J]. 广州环境科学, 2006, 21 (2): 14-18 [Li YX, Zhang XL, Jiang AX, Meng XZ, Han XY. Application of cold-adapted microorganisms in the treatment of sewage at low temperature [J]. Guanzhou Environ Sci, 2006, 21 (2): 14-18]
3 Kim DJ, Lee DI, Keller J. Effect of temperature and free ammonia on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifying bacterial community by fish [J]. Bioresour Technol, 2006, 97: 459-468
4 Yao S, Ni JR, Chen Q, Borthwick AGL. Enrichment and characterization of a bacteria consortium capable of heterotrophic nitrification and aerobic denitrification at low temperature [J]. Bioresour Technol, 2013, 127: 151-157
5 姜体胜, 杨琦, 尚海涛, 甘一萍, 白宇, 王洪臣. 温度和pH值对活性污泥法脱氮除磷的影响[J]. 环境工程学报, 2007, 1 (9): 10-14 [Jiang TS, Yang Q, Shang HT, Gan YP, Bai Y, Wang HC. Effects of temperature and pH on nitrogen and phosphorus removal by activated sludge [J]. Chin J Environ Eng, 2007, 1 (9):10-14]
6 Shan H, Obbaed JP. Ammonia removal from freshwater using nitrifying bacteria enriched from a seawater aquaculture pond [J]. Biotechonlogy, 2003, 25 (17): 1469-1471
7 王小菊, 何春平,王震, 邢传宏, 宋安东, 陈红歌. 高效硝化细菌的筛选及特性研究[J]. 中国环境科学, 2013, 33 (2): 286-292 [Wang XJ, He CP, Wang Z, Xing CH, Song AD, Chen HG. Screening and characteristics of an efficient nitrobacterium [J]. China Environ Sci, 2013, 33 (2): 286-292]
8 韩洪军, 黄集华, 马文成. 低温对于脱氮效果影响的试验研究[J]. 现代化工, 2005 (S1): 171-177 [Han HJ, Huang JH, Ma WC. Experimental study on nitrogen removing efficiency at low temperature [J]. Mod Chem Ind, 2005 (S1): 171-177]
9 王晓东, 毕学军, 初正崑, 陈栋, 刘长青. 反应温度变化对活性污泥沉降性能的影响分析[J]. 中国给水排水, 2013, 29 (23): 128-131 [Wang XD, Bi XJ, Chu ZK, Chen D, Liu CQ. Impact of reaction temperature on settleability of activated sludge [J]. China Water Wastewater, 2013, 29 (23): 128-131]
10 陈皓文, 高爱国. 中国极地微生物学调查研究进展[J]. 极地研究, 2005, 17 (4): 299-307 [Chen HW, Gao AG. Progress on investigation and research of polar microbiology in China [J]. Chin J Polar Res, 2005, 17 (4): 299-307]
11 Zeng YX, Zou Y, Chen B. Phylogenetic diversity of sediments bacteria in the northern Bering Sea [J]. Polar Biol, 2011, 34 (6): 907-919
12 Chevalier P, Proulx D, Lessard P. Nitrogen and phosphorus removal by high latitude mat-forming cyanobacteria for potential use in tertiary wastewater treatment [J]. J Appl Phycol, 2000, 12: 105-112
13 徐建, 张德纯, 周广. 微生物在城市污水处理系统中的应用与展望[J]. 中国微生态学杂志, 2006, 18 (1): 75-77 [Xu J, Zhang DC, Zhou G. Microbial application and prospect in urban sewage treatment system [J]. Chin J Microecol, 2006, 18 (1): 75-77]
14 汪苹, 王斌, 刘军. 同步硝化反硝化处理氨氮废水过程中气态脱氮产物的研究[J]. 环境科学研究, 2005, 18 (5): 67-71 [Wang P, Wang B, Liu J. Study on gaseous nitrogen compounds emitted during ammoniac wastewater SND process [J]. Res Environ Sci, 2005, 18 (5): 67-71]
15 王桢, 李阳, 车帅, 林学政. 北极海洋沉积物中可培养细菌及其多样性分析[J]. 海洋学报, 2014, 36 (10): 116-123 [Wang Z, Li Y, Che S, Lin XZ. Diversity analysis of culturable bacteria isolated from marine sediments of Arctic [J]. Acta Oceanol Sin, 2014, 36 (10): 116-123]
16 Kim OS, Cho YJ, Lee K. Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species [J]. Intern J Syst Evol Microbiol, 2012, 62 (Pt 3): 716-721
17 许学伟, 吴敏, 黄伟达. 阿尔金山盐湖极端嗜盐古生菌的分离及16S rRNA序列分析[J]. 浙江大学学报, 2004, 38 (2): 508-511 [Xu XW, Wu M, Huang WD. Isolation and 16S rDNA sequence analysis of extremely halophilic archaea from saline in Altun region [J]. J Zhejiang Univ (Eng Sci), 2004, 38 (4): 508-512]
18 Canion A, Prakash O, Green SJ. Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments [J]. Environ Microbiol, 2013, 15 (5): 1606-1618
19 张淑梅, 姜威, 李晶, 孟利强, 曹旭, 陈静宇, 赵晓宇, 张云湖. 低温氨氮降解菌的筛选及降解能力研究[J]. 生物技术, 2014, 24 (1): 89-92 [Zhang SM, Jiang W, Li J, Meng LQ, Cao X, Chen JY, Zhao XY, Zhang YH. Isolation and ammonium removal of a nitrifier at low temperature [J]. Biotechnology, 2014, 24 (1): 89-92]
20 辛玉峰, 曲晓华, 袁梦冬, 荆延德. 一株异养硝化反硝化不动杆菌的分离鉴定及脱氮活性[J]. 微生物学报, 2011, 51 (12): 1646-1654 [Xin YF, Qu XH, Yuan MD, Jing YD. Isolation and identification of a heterotrophic nitrifying and aerobic denitrifying Acinetobacter sp. YF14 and its denitrification activity [J]. Acta Microbiol Sin, 2011, 51 (12): 1646-1654]
21 潘丹, 黄巧云, 陈雯莉. 两株异养硝化细菌的分离鉴定及其脱氮特性[J]. 微生物学报, 2011, 51 (10): 1382-1389 [Pan D, Huang QY, Chen WL. Screening and identification of two heterotrophic nitrifying bacteria and characterization of their capacity for nitrogen removal [J]. Acta Microbiol Sin, 2011, 51 (10): 1382-1389]
22 吴建江, 王兆阳, 许培雅. 一株高效异养硝化菌的分离、鉴定及其氨氮去除特性[J]. 中国环境科学, 2013, 33 (7): 1309-1315 [Wu JJ, Wang ZY, Xu PY. Isolation and identification of an efficiently heterotrophic nitrifier, and its removal characterization of ammonia nitrogen [J]. China Environ Sci, 2013, 33 (7): 1309-1315]
23 Shapovalova AA, Khijniak TV, Tourova TP. Heterotrophic denitri?cation at extremely high salt and pH by haloalkaliphilic Gamma-proteobacteria from hypersaline soda lakes. Extremophiles, 2008, 12: 619-625
24 孙雪梅, 李秋芬, 张艳, 刘淮德, 赵俊, 曲克明. 一株海水异养硝化-好氧反硝化菌系统发育及脱氮特性[J]. 微生物学报, 2012, 52 (6): 687-695 [Sun XM, Li QF, Zhang Y, Liu HD, Zhao J, Qu KM. Phylogenetic analysis and nitrogen removal characteristics of a heterotrophic nitrifying-aerobic denitrifying bacteria strain from marine environment [J]. Acta Microbiol Sin, 2012, 52 (6): 687-695]

Memo

Memo:
-
Last Update: 2017-04-25