|Table of Contents|

Changes of lignocellulolytic enzymes and material components in different compost formulas during the production of Agaricus bisporus(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017 06
Page:
1035-1041
Research Field:
Articles
Publishing date:

Info

Title:
Changes of lignocellulolytic enzymes and material components in different compost formulas during the production of Agaricus bisporus
Author(s):
QIN Gaijuan1 WANG Xiao1 CHEN Qingjun1**& ZHANG Guoqing2
1 College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China 2 College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
Keywords:
Agaricus bisporus straw compost extracellular enzyme activity lignocelluloses
CLC:
S141.4 : Q938.1
PACS:
DOI:
10.3724/SP.J.1145.2017.01019
DocumentCode:

Abstract:
In order to investigate the utilization of lignocellulose during Agaricus bisporus production, three formulas were studied. Wheat straw (FMS), corn stalks (FCS), and reservoir weeds (FRW) were used as principal carbon sources. During the composting and fruiting stages, activities of main lignocellulolytic enzymes and relative proportions of lignocellulosic components (lignin, cellulose, and hemicellulose) were assayed. After harvest, mushroom yields in different formulas were counted. The results showed that total cellulase and xylanase activities in FWS continuously increased and stayed at a high level during the fruiting period. Total cellulase activities of FCS and FRW were ~1 U/g during the composting period, followed by a sharp increase and soft decrease during the fruiting period. Xylanase activity of FCS and FRW stayed in a range of 7.97–23.85 U/g. Laccase activities in the three formulations were unmeasurable during the composting stage, reached the highest level in the spawning period, and then rapidly decreased in the fruiting period. During the composting period, relative contents of cellulose and hemicellulose in the three formulations significantly decreased, while relative content of lignin did not change. The relative contents of lignin and hemicellulose decreased obviously in the spawning period and fruiting period. Total yields of the three formulations were FWS (30.00 kg/m2) > FCS (17.21 kg/m2) > FRW (16.67 kg/m2). During composting, microbial communities and physicochemical properties had strong effects on cellulose and hemicellulose degradation. On the other hand, lignin was mainly used by A. bisporus mycelia at the spawning stage. In the spawning and fruiting period, A. bisporus mycelia mainly used lignin and hemicellulose. Based on the present study, FWS is the best culture formula for mushroom production, while formulas FCS and FRW need further improvements.

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Last Update: 2017-12-25