|Table of Contents|

Comparison of two population investigation methods for wild giant pandas(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017 06
Page:
1142-1147
Research Field:
Articles
Publishing date:

Info

Title:
Comparison of two population investigation methods for wild giant pandas
Author(s):
ZHANG Jindong1** LI Yujie1 WANG Yujun1 OUYANG Zhiyun2 QIAO Maiju3 & ZHANG Hemin3
1 College of Life Sciences, China West Normal University, Nanchong 637002, China 2 State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-environmental Science, Chinese Academy of Sciences, Beijing 100085, China 3 China Conservation and Research Center for the Giant Panda, Wolong Nature Reserve, Wenchuan 623006, China
Keywords:
giant panda population number distance-bamboo stem fragments method molecular biology method Wolong Nature Reserve
CLC:
Q959.838.08 : Q3
PACS:
DOI:
10.3724/SP.J.1145.2017.01010
DocumentCode:

Abstract:
The giant panda (Ailuropoda melanoleuca) is a flagship species of wildlife protection. Much attention has been paid to its survival status and conservation. Population size serves as the basis for giant-panda conservation. So far, the accuracy, operability, and cost of two main survey methods of panda population estimation (distance-bamboo stem fragments method and molecular biological method) have been controversial. We estimated the panda population using both the distance-bamboo stem fragments method and molecular biological method in the main panda distribution areas of the Wolong Nature Reserve in China, and compared the advantages and disadvantages of the two methods. The results showed a more than 50% lower population estimate for the distance-bamboo stem fragments method compared to the molecular biological method. To improve the sensitivity of the methods for identifying panda individuals and considering the characteristics of giant panda behavior, we propose to reduce the distance threshold value for identifying different individuals in the high-density area of a giant-panda distribution.

References

1 四川省林业厅. 四川的大熊猫——四川省第四次大熊猫调查报告[M]. 成都: 科学出版社, 2015 [Sichuan Provincial Forestry Department 2015. The Giant Panda of Sichuan - The Fourth Giant Panda Survey of Sichuan Province [M]. Chengdu: Sichuan Science and Technology Press, 2015]
2 胡锦矗, 夏勒. 卧龙的大熊猫[M]. 成都: 四川科技出版社, 1985 [Hu JC, Schaller GB. Wolong’s Giant Panda [M]. Chengdu: Sichuan Science and Technology Publishing House, 1985]
3 国家林业局. 全国第三次大熊猫调查报告[M]. 北京: 科学出版社, 2006 [State Forestry Administration. The Third National Survey Report on the Giant Panda in China [M]. Beijing: Science Press, 2006]
4 史雪威, 张晋东, 欧阳志云. 野生大熊猫种群数量调查方法研究进展. 生态学报, 2016, 36 (23): 7528-7537 [Shi XW, Zhang JD, Ouyang ZY. Research progress on population investigation methods for wild giant panda [J]. Acta Ecol Sin, 2016, 36 (23): 7528-7537]
5 魏辅文, 饶刚, 李明, 方盛国, 冯祚建. 分子粪便学及其应用-可靠性、局限性和展望[J]. 兽类学报, 2001, 21 (2): 143-152 [Wei FW, Rao G, Li M, Fang SG, Feng ZJ. Molecular scatology and its application reliability limitation and prospect [J]. Acta Theriol Sin, 2001, 21 (2): 143-152]
6 曹玫, 杨玉华, 张义正, 王喜忠, 王亚军, 宋云芳, 费立松, 何光昕. 大熊猫基因组微卫星DNA的分离与序列分析[J]. 应用与环境生物学报, 2001, 7 (3): 277-280 [Cao M, Yang YH, Zhang YZ, Wang XZ, Wang YJ, Song YF, Fei LS, He GX. Isolation and characterization of microsatellite DNA from giant panda (Ailuropoda melanoleuca) genome [J]. Chin J Appl Environ Biol, 2001, 7 (3): 277-280]
7 魏荣平, 张贵权, 王鹏彦, 李德生, 曹家林. 大熊猫粪便中竹子咬节长短与年龄和种群数量关系[J]. 生态学报, 2003, 23 (10): 2153-2162 [Wei RP, Zhang GQ, Wang PY, Li DS, Cao JL. Relation of bite size in feces with age and population number of Giant Panda (Ailuropoda melanoleuca) [J]. Acta Ecol Sin, 2003, 23 (10): 2153-2162]
8 潘文石, 吕植, 朱小健, 王大军, 王昊, 龙玉, 付达莉, 周欣. 继续生存的机会[M]. 北京: 北京大学出版社, 2001 [Pan WS, Lv Z, Zhu XJ, Wang DJ, Wang H, Long Y, Fu DL, Zhou X. A Chance for Lasting Survival [M]. Beijing: Beijing University Press, 2001]
9 Rodgers AR, Rempel RS, Abraham KF. A GPS-based telemetry system [J]. Wildl Soc Bull, 1996, 24: 559-566
10 Zhang J, Hull V, Huang J, Zhou S, Xu W, Yang H, McConnell WJ, Li R, Liu D, Huang Y. Activity patterns of the giant panda (Ailuropoda melanoleuca) [J]. J Mammal, 2015, 96 (6): 1-12
11 Zhang Z, Sheppard J K, Swaisgood RR, Wang G, Nie Y, Wei W, Zhao N, Wei F. Ecological scale and seasonal heterogeneity in the spatial behaviors of giant pandas [J]. Integr Zool, 2014, 9 (1): 46-60
12 Hull V, Zhang J, Zhou S, Huang J, Li R, Liu D, Xu W, Huang Y, Ouyang Z, Zhang H. Space use by endangered giant pandas [J]. J Mammal, 2015, 96 (1): 230-236
13 谭迎春, 欧阳志云, 张和民. 卧龙自然保护区生物多样性空间特征研究[J]. 中国生物圈保护区, 1995 (3): 19-24 [Tan YC, Ouyang ZY, Zhang HM. Spatial characteristics of bio-diversity in Wolong Nature Reserve [J]. China Biosphere Reserve, 1995 (3): 19-24]
14 Zhang J, Hull V, Ouyang Z, Li R, Connor T, Yang H, Zhang Z, Silet B, Zhang H, Liu J. Divergent responses of sympatric species to livestock encroachment at fine spatiotemporal scales [J]. Biol Conserv, 2017, 209: 119-129
15 黄杰. 大熊猫微卫星标记标准化方法的建立与应用[D]. 成都: 四川大学, 2015 [Huang J. The development and the applications of universal marker system based on microsatellites of giant panda (Ailuropoda melanoleuca) [D]. Chengdu: Sichuan University, 2015]
16 尹玉峰, 王昊, 陈艾, 刘国琪. 对大熊猫数量调查方法中咬节区分机制的准确性评价[J]. 生物多样性, 2005, 13 (5): 439-444 [Yin YF, Wang H, Chen A, Liu GQ. Accuracy assessment of the bamboo stem fragment distinguishing method used in panda population surveys [J]. Biodiv Sci, 2005, 13 (5): 439-444]
17 Li RQ, Fan W, Tian G, Zhu HM, He L, Cai J, Huang QF, Cai QL, Li B, Bai YQ, Zhang ZH, Zhang YP, Wang W, Li J, Wei FW, Li H, Jian M, Li JW, Zhang ZL, Nielsen R, Li DW, Gu WJ, Yang ZT, Xuan ZL, Ryder OA, Leung FCC, Zhou Y, Cao JJ, Sun X, Fu YG, Fang XD, Guo XS, Wang B, Hou R, Shen FJ, Mu B, Ni PX, Lin RM, Qian WB, Wang GD, Yu C, Nie WH, Wang JH, Wu ZG, Liang HQ, Min JM, Wu Q, Cheng SF, Ruan J, Wang MW, Shi ZB, Wen M, Liu BH, Ren XL, Zheng HS, Dong D, Cook K, Shan G, Zhang H, Kosiol C, Xie XY, Lu ZH, Zheng HC, Li YR, Steiner CC, Lam TTY, Lin SY, Zhang QH, Li GQ, Tian J, Gong TM, Liu HD, Zhang DJ, Fang L, Ye C, Zhang JB, Hu W B, Xu AL, Ren YY, Zhang GJ, Bruford MW, Li QB, Ma LJ, Guo YR, An N, Hu YJ, Zheng Y, Shi YY, Li ZQ, Liu Q, Chen YL, Zhao J, Qu N, Zhao SC, Tian F, Wang XL, Wang HY, Xu LZ, Liu X, Vinar T, Wang YJ, Lam TW, Yiu SM, Liu SP, Zhang HM, Li DS, Huang Y, Wang X, Yang GH, Jiang Z, Wang J Y, Qin N, Li L, Li J X, Bolund L, Kristiansen K, Wong GKS, Olson M, Zhang XQ, Li SG, Yang HM, Wang J, Wang J. The sequence and de novo assembly of the giant panda genome [J]. Nature, 2010, 463 (7279): 311-317
18 方盛国, 冯文和, 张安居, 李绍昌, 余建秋, 何光昕, 费立松. 大熊猫父权认定的DNA指纹圈分析[J]. 兽类学报, 1997, 17 (2): 92-99 [Fang SG, Feng WH. Zhang AJ, Li SC, Yu JQ, He GX, Fei LS. DNA fingerprinting analysis on the paternity determination of giant panda [J]. Acta Theriolo Sin, 1997, 17 (2): 92-99]
19 Zhan XJ, Li M, Zhang ZJ, Goossens B, Chen YP, Wang HJ, Bruford MW, Wei FW. Molecular censusing doubles giant panda population estimate in a key nature reserve [J]. Curr Biol, 2006, 16 (12): R451-R452
20 Wei F, Hu Y, Zhu L, Bruford MW, Zhan X, Zhang L. Black and white and read all over: the past, present and future of giant panda genetics [J]. Mol Ecol, 2012, 21 (23): 5660-5674
21 张晋东. 人类与自然干扰下大熊猫空间利用与活动模式研究[D]. 北京: 中国科学院研究生院, 2012 [Zhang JD. Activity patterns and space use of the giant panda in relationship to human and natural disturbance [D]. Beijing: Graduate University of Chinese Academy of Sciences, 2012]
22 Hull V, Zhang JD, Zhou SQ, Huang JY, Li RG, Liu D, Xu WH, Huang Y, Ouyang ZY, Zhang HM, Liu JG. Habitat use and selection by giant pandas [J]. PLoS ONE, 2016, 11 (9): 1-18
23 黄乘明, 胡锦矗. 野外大熊猫调查方法的研究[J]. 西华师范大学学报:自然科学版, 1989, 10 (1): 93-99 [Huang CM, Hu JC. The research of wild giant panda’s survey methods [J]. J China West Norm Univ (Nat Sci), 1989, 10 (1): 93-99]
24 张泽钧, 杨旭煜, 吴华, 李明, 魏辅文. 大熊猫粪便宽径与咬节平均长度的关系[J]. 兽类学报, 2005, 25 (4): 351-354 [Zhang ZJ, Yang XY, Wu H, Li M, Wei FW. Correlation between fecal width and average length of bamboo stem fragment of the giant panda (Ailuropoda melanoleuca) [J]. Acta Theriol Sini, 2005, 25 (4): 351-354]

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Last Update: 2017-12-25