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Optimization of somatic embryogenesis system from embryogenic callus and plant regeneration from transformed HPTresistant calli of litchi (Litchi chinensis Sonn.)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2003 Vol.09 No.2
Page:
131-136
Research Field:
Reviews
Publishing date:

Info

Title:
Optimization of somatic embryogenesis system from embryogenic callus and plant regeneration from transformed HPTresistant calli of litchi (Litchi chinensis Sonn.)
Author(s):
LAI Zhongxionget al.
Keywords:
Keywords litchi embryogenic callus resistant callus somatic embryogensis plant regeneration
CLC:
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PACS:
DOI:
-
DocumentCode:

Abstract:
Abstract The factors affecting the regeneration of litchi (Litchi chinensis Sonn.) embryogenic calli derived from immature embryos were optimized and somatic embryogenesis at high frequency (100%) was obtained on the MS medium with 5 mg L-1 ZT, 60 g L-1 sucrose and 10 g L-1 agar. The problems about somatic embryo maturation and germination were also discussed, then an intact litchi regeneration system was established, and the somatic embryos normally matured on the MS medium with 10% coconut water and 6% sucrose and germinated on the medium free of phytohormones, which provided a good regeneration system for litchi transformation. Using the system, regeneration of litchi resistant calli from particle bombardment was performed and a number of plantlets were obtained. Fig 1, Tab 7, Ref 11

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Last Update: 2001-04-23