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Effect of arginine esterase from Agkistrodon halys ussuriensis venom on CHL cell proliferation inhibition and chromosome aberration(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
Vol.08 No.6
Page:
636-639
Research Field:
Reviews
Publishing date:

Info

Title:
Effect of arginine esterase from Agkistrodon halys ussuriensis venom on CHL cell proliferation inhibition and chromosome aberration
Author(s):
HU Jianraoet al.
(1School of Life Science, Hangzhou Teachers College, Hangzhou310036, China) (2 College of Life Science, Zhejiang University, Hangzhou310027, China) (3 Biology Deparment, Wanxi Teachers College, Liu an, Anhui237000, China)
Keywords:
Keywords snake venom arginine esterase CHL cell cell proliferation inhibition chromosome aberration
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
Abstract The effect of arginine esterase (an effective component of ahylysantinfarctase) on CHL cell growth inhibition and chromosome aberration in vitro was studied. The concentration of arginine esterase was indicated as enzyme activity (U). Cell growth inhibition test and cell chromosome aberration test were studied by two methods including direct method and activating metablism method. The arginine esterase concentration in the experiment was:1.0×10-3 U/mL, 0.5×10- 3 U/mL and 0.25×10-3 U/mL. 1.0 ×10-3 U/mL was about 100 times higher than clinical concentration. Cell growth inhibition was tested by cell sticking cultivation, ethanol fixation, crystal violet staining and monolayer cell density assaying. Chromosome aberration was tested by collecting metaphase dividing cells at 24 h and 48 h after cultivation, low solution infiltration, fixation, and Giemas staining. The chromosome structure and polyploid of 100 metaphase cells were studied and the polypoid cells and aberration cells were counted. The results showed that arginine esterase could not affect CHL cell growth and chromosome aberration at the above concentrations.Tab 2, Ref 10

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Last Update: 2002-12-19