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Structure Prediction and Function Analysis of TAK Kinase in Arabidopsis(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2009 05
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Structure Prediction and Function Analysis of TAK Kinase in Arabidopsis
JIANG Jiahong WANG Dong HU Yuan & DU Linfang
(1Key Laboratory of Ministry of Education for Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041, China)
TAK phosphorylation LHCII structure of core domain homologous modeling antibody

TAK1, TAK2 and TAK3 belong to TAK kinase family and are nuclear-encoded kinases. TAK1 is involved in the phosphorylation of LHCII and the state transitions. Few studies on the structure or function of TAKs have been done because they are membrane combined proteins. In this research, TAKs were studied using a systematical bioinformatical method and all the members of TAK kinase family were found with a hydrophobic N-terminal connected with a transmembrane region located in thylakoid membrane and a hydrophilic C-terminal (kinase catalytic domains) on the stromal side of thylakoid. Lots of sequences which contained the same TyrKc kinase catalytic domains with a similarity more than 30% were found in PDB, and three dimensional structures of core domains of TAK1, TAK2 and TAK3 were then predicted by using homologous modeling method. The analysis of the relationship between the structure and function of TAKs provided the foundation for further studying the functions of TAK1, TAK2 and TAK3. We designed an antibody on the base of prediction of the three dimensional structures, and its western blotting showed that there was an immunoaffinity reaction. Fig 5, Tab 1, Ref 15


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Last Update: 2009-10-26