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Verification of Nodulation Ability of Strains Isolated from Mimosa spp. Nodules Using Whole Cell Protein SDS-PAGE Molecular Marker Method(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2009 05
Page:
719-723
Research Field:
T & M
Publishing date:

Info

Title:
Verification of Nodulation Ability of Strains Isolated from Mimosa spp. Nodules Using Whole Cell Protein SDS-PAGE Molecular Marker Method
Author(s):
LIU Xiaoyun ZHANG Bin WU Wei & LI Qiaoxian
(1College of Life Sciences, Hebei University, Key Laboratory of Microbial Diversity Research and Application of
Hebei Province, Baoding 071002, Hebei, China)
(2College of Resources, Southwest Forestry College, Key Laboratory of Biodiversity Conservation in Southwest China of
State Forestry Administration, Kunming 650224, China)
(3College of Conservation Biology, Southwest Forestry College, Kunming 650224, China)
(4Academy of Grassland and Animal Science of Yunnan, Kunming 650212, China)
Keywords:
Mimosa rhizobia molecular marker whole cell protein pattern SDS-PAGE
CLC:
S154.381
PACS:
DOI:
10.3724/SP.J.1145.2009.00719
DocumentCode:

Abstract:
The nodules of Mimosa spp. were collected from some areas in Yuanjiang and Xishuangbanna, Yunnan, China. Bacteria were isolated from these nodules with modified medium, and 40 strains were selected to carry out the nodulation test. The result showed that 38 strains could nodulate M. pudica. The nodulation rate was approximately 95%. 38 re-isolated strains and their whole cell protein profiles were obtained. The clustering analysis demonstrated that 26 re-isolated strains had a protein pattern identical to that of their original strains at the similarity of 100%, indicating that these 26 strains could establish symbiosis with M. pudica. The whole cell protein profiles of 12 strains were different from those of the re-isolated strains, indicating that the nodules were not formed by them and further research is needed. This study shows that the whole cell protein SDS-PAGE can be used in inoculation test, and it is a time-saving and accurate molecular marker to identify rhizobia, especially for characterizing plenty of isolates from nodules of various plants. Also, this study preliminarily reveals that the competitive nodulation ability differs among rhizobial strains. Fig 2, Ref 28

References

1 Wang LL, Wang ET, Liu J, Li Y, Chen WX. Endophytic occupation of root nodules and roots of Melilotus dentatus by Agrobacterium tumefaciens. Microbial Ecol, 2006, 52: 436~443
2 Wang ET, Rogel MA, Garcia-de los Santos A, Martinez-Romero J, Cevallos MA. Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis. Int J Syst Evol Microbiol, 1999, 49: 1479~1491
3 Chen WM, Laevens S, Lee TM, Coenye T, De Vos P, Mergeay M, Vandamme P. Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol, 2001, 51: 1729~1735
4 Chen WM, Moulin L, Bontemps C, Vandamme P, Bena G, Boivin-Masson C. Legume symbiotic nitrogen fixation by β-proteobacteria is widespread in nature. J Bacteriol, 2003b, 185 (24): 7266~7272
5 Elliott GN, Chen WM, Chou JH, Wang HC, Sheu SY, Perin L, Reis VM, Moulin L, Simon MF, Bontemps C, Sutherland JM, Bessi R, De Faria SM, Trinick MJ, Prescott AR, Sprent JI, James SEK. Burkholderia phymatum is a highly effective nitrogenfixing symbiont of Mimosa spp. and fixes nitrogen ex planta. New Phytol, 2007, 173: 168~180
6 Chen WM, James EK, Coenye T, Chou JH, Barrios E, De Faria SM, Elliott GN, Shih YS, Sprent JI, Vandamme P. Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int J Syst Evol Microbiol, 2006, 58: 1847~1851
7 Chen WM, De Faria SM, James EK, Elliott GN, Lin KY, Chou JH, Sheu SY, Cnockaert M, Sprent JI, Vandamme P. Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella. Int J Syst Evol Microbiol, 2007, 57: 1055~1059
8 Chen WM, De Faria SM, Chou JH, James EK, Elliott GN, Sprent JI, Bontemps C, Young JP, Vandamme P. Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia. Int J Syst Evol Microbiol, 2008, 58: 2174~2179
9 Parker MA, Wurtz AK, Payter Q. Nodule symbiosis of invasive Mimosa pigra in Australia and in ancestral habitats: A comparative analysis. Biol Inv, 2007, 9: 127~138
10 Chen WM, James EK, Prescott AR, Kierans M, Sprent JI. Nodulation of Mimosa spp. by the β-proteobacterium Ralstonia taiwanensis. Mol Pl Micr Interact, 2003a, 16: 1051~1061
11 Chen WM, De Faria SM, Straliotto R, Pitard RM, Simoes-Araujo JL, Chou JH, Chou YJ, Barrios E, Prescott AR, Elliott GN, Sprent JI, Young JPW, James EK. Proof that Burkholderia strains form effective symbioses with legumes: A study of novel mimosa-nodulating strains from South America. Appl Environ Microbiol, 2005a, 71 (11): 7461~7471
12 Chen WM, James EK, Chou JH, Shih YS, Yang SZ, Sprent JI. β-rhizobia from Mimosa pigra, a newly discovered invasive plant in Taiwan. New Phytol, 2005b, 168: 661~675
13 Tan ZY, Hurek T, Vinuesa P, Muller P, Ladha JK, Reinhold-Hurek B. Specific detection of Bradyrhizobium and rhizobium strains colonizing rice (Oryza sativa) roots by 16S-23S ribosomal DNA intergenic spacer-targeted PCR. Appl Environ Microbiol, 2001, 67 (8): 3655~3664
14 Pan F (潘峰), Wang P (王平), Hu ZJ (胡正嘉), He SJ (何绍江), Feng XM (冯新梅). Phylogenic study of rhizobia strains isolated from rice bean (Vignaum bellata L.) in Hubei by 16S-23S rDNA RFLP and 16S rRNA gene sequencing. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (1): 78~82
15 Zeng ZH (曾昭海), Chen WX (陈文新), Hu YG (胡跃高), Sui XH (隋新华), Chen DM (陈丹明). Study on competitive nodulation ability of Rhizobium melilotiin field test by using RAPD Molecular Marker Method. Acta Ecol Sin (生态学报), 2004, 24 (7): 1341~1345
16 Gao JL (高俊莲), Chen WX (陈文新), Kristina L, Terefework Z. Study on genotypic diversity of the rhizobia isolated from Astragalus adsurgens by AFLP fingerprinting. Chin J Appl Environ Biol (应用与环境生物学报), 1999, 5(4): 387~395
17 Tan ZY (谭志远), Chen WX (陈文新). SDS-PAGE of whole cell protein of new rhizobial groups and 16S rDNA sequencing of their representatives. Chin J Appl Environ Biol (应用与环境生物学报), 1998, 4 (1): 65~69
18 Vincent JM. A Manual for the Practical Study of Root Nodule Bacteria. Edinburgh: Blackwell Scientific Pubilications, 1970
19 Gu JG (顾金刚). Rulers for the Collection Characteration Preservation Management of Microorganism Resources No. 1. Beijing, China (北京): Chinese Agricultural Press, 2005
20 Somasegaran P, Hoben HJ. Handbook for Rhizobia. Methods in Legume-Rhizobium Technology. New York: Springer – Verlag, 1994
21 Guo YJ (郭尧君). Experimental Techniques of Protein Electrophoresis. Beijing, China (北京): Science Press, 1999
22 Liu XY, Wang ET, Li Y, Chen WX. Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of China. Arch Microbiol, 2007, 188: 1~14
23 Chen DM (陈丹明). Screening and molecular labeling of highly effective symbiotic rhizobia on alfalfa: [Master’s Degree Dissertation]. Beijing, China (北京): China Agricultural University, 2002
24 Qi J (祁娟). Screening endogenous rhizobia from alfalfa seeds and their promoting alfalfa seedlings growth property: [Master’s Degree Dissertation]. Lanzhou, China (兰州): Gansu Agricultural University, 2006
25 Benhizia Y, Benhizia H, Benguedouar A, Muresu R, Giacomini A, Squartini A. Gamma proteobacteria can nodulate legumes of the genus Hedysarum. Syst Appl Microbiol, 2004, 27: 462~468
26 Li JH, Wang ET, Chen WF, Chen WX. Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biol Biochem, 2008, 40: 238~246
27 Muresu R, Polone E, Sulas L, Baldan B, Tondello A, Delogu G, Cappuccinelli P, Alberghini S, Benhizia Y, Benhizia H, Benguedouar A, Mori B, Calamassi R, Dazzo FB, Squartini A. Coexistence of predominantly nonculturable rhizobiawith diverse, endophytic bacterial taxa within nodules of wild legumes. Microbial Ecol, 2008, 63: 383~400
28 Liu XY (刘晓云), Chen WX (陈文新), Zhang B (张斌). β-proteorhizobia and nonrhizobial species—A review. Acta Microbiol Sin (微生物学报), 2008, 48 (10): 1408~1412

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Last Update: 2009-10-26