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Verification of Nodulation Ability of Strains Isolated from Mimosa spp. Nodules Using Whole Cell Protein SDS-PAGE Molecular Marker Method(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2009 05
Research Field:
T & M
Publishing date:


Verification of Nodulation Ability of Strains Isolated from Mimosa spp. Nodules Using Whole Cell Protein SDS-PAGE Molecular Marker Method
LIU Xiaoyun ZHANG Bin WU Wei & LI Qiaoxian
(1College of Life Sciences, Hebei University, Key Laboratory of Microbial Diversity Research and Application of
Hebei Province, Baoding 071002, Hebei, China)
(2College of Resources, Southwest Forestry College, Key Laboratory of Biodiversity Conservation in Southwest China of
State Forestry Administration, Kunming 650224, China)
(3College of Conservation Biology, Southwest Forestry College, Kunming 650224, China)
(4Academy of Grassland and Animal Science of Yunnan, Kunming 650212, China)
Mimosa rhizobia molecular marker whole cell protein pattern SDS-PAGE

The nodules of Mimosa spp. were collected from some areas in Yuanjiang and Xishuangbanna, Yunnan, China. Bacteria were isolated from these nodules with modified medium, and 40 strains were selected to carry out the nodulation test. The result showed that 38 strains could nodulate M. pudica. The nodulation rate was approximately 95%. 38 re-isolated strains and their whole cell protein profiles were obtained. The clustering analysis demonstrated that 26 re-isolated strains had a protein pattern identical to that of their original strains at the similarity of 100%, indicating that these 26 strains could establish symbiosis with M. pudica. The whole cell protein profiles of 12 strains were different from those of the re-isolated strains, indicating that the nodules were not formed by them and further research is needed. This study shows that the whole cell protein SDS-PAGE can be used in inoculation test, and it is a time-saving and accurate molecular marker to identify rhizobia, especially for characterizing plenty of isolates from nodules of various plants. Also, this study preliminarily reveals that the competitive nodulation ability differs among rhizobial strains. Fig 2, Ref 28


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Last Update: 2009-10-26