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Characterization of the Complexation Between Al3+ and Extracellular Polymeric Substances Prepared from Alga-bacteria Biofilm(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2009 03
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Characterization of the Complexation Between Al3+ and Extracellular Polymeric Substances Prepared from Alga-bacteria Biofilm
LIU JingZHANG DaoyongPAN XiangliangWANG Liying
1State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China
2Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China
3Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
extracellular polymeric substance (EPS) Al3+ three-dimensional excitation emission matrix (3DEEM) fluorescence quenching Fourier transform infrared spectroscopy biofilm conditional stability constant
X172 : Q936

Three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM) and fourier transform infrared (FTIR) spectroscopy were used to study the extracellular polymeric substances (EPS) produced by alga-bacteria biofilm. Three excitation/emission (Ex/Em) fluorescence peaks at Ex/Em 225~235/300~330 nm (Peak A), 275~280/325~330 nm (Peak B), and 335/432~434 nm (Peak C) were identified in the 3DEEM, respectively. Peaks A and B were referred to as protein-like fluorescence and peak C as humic-like fluorescence. The fluorescence intensity at both peaks A and B decreased as Al3+ concentration increased. The values of logK (conditional stability constant) for peaks A and B were 5.89 and 6.95, respectively. It was also found that solution pH strongly affected the fluorescence intensity at peaks A and B for the Al3+-EPS complexation. The fluorescence intensity at peaks A and B increased consistently with solution pH increasing from 2 to 4, decreased with solution pH increasing from 4 to 7, and increased again with solution pH increasing from 7 to 11. FTIR analysis demonstrated that —NH— and C==O groups in EPS were responsible for binding with Al3+. Fig 6, Ref 21


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Last Update: 2009-07-03