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An improved 1DSDSPAGE method for identification of wheat waxy protein(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2000 Vol.06 No.5
Page:
487-489
Research Field:
Reviews
Publishing date:

Info

Title:
An improved 1DSDSPAGE method for identification of wheat waxy protein
Author(s):
PAN Zhifenet al.
Keywords:
Keywords wheat waxy protein 1DSDSPAGE
CLC:
-
PACS:
DOI:
-
DocumentCode:

Abstract:
Abstract The methods for extraction of wheat waxy protein and silver staining of gel are improved. When extracting waxy protein, crushed seeds are dipped into washing buffer for 2 h rather than distilled water for overnight, moreover, washing granule repeatedly with washing buffer and microfuging are omitted; and gel is stained with rapid silver staining. The results show that the improved 1DSDSPAGE method for identification of wheat waxy protein is more convenient, rapid, cheap and available, especially for bulks of wheat. Fig 1, Ref 5

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Last Update: 2002-11-05