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Structure analysis and function characterization of kat A and kat B gene from Deinococcus radiodurans(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
Vol.07 No.2
Page:
163-169
Research Field:
Reviews
Publishing date:

Info

Title:
Structure analysis and function characterization of kat A and kat B gene from Deinococcus radiodurans
Author(s):
LU Xinget al.
(Beijing Institute of Radiation Medicine, Beijing100850, China) (1 Postgraduate School, Chinese University of Science and Technology, Beijing100039, China)
Keywords:
Keywords Deinococcus radiodurans catalase kat A kat B
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
Abstract By using a 672 bp length catalase gene fragment as probe which had been amplified from D. radiodurans genome DNA by degenerate primers PCR reaction and bioimformatical analysis, two complete coding sequences of the catalase were determined from the database of D. radiodurans genome, which were then designated as kat A and kat B gene. The kat A and kat B gene were 2 277 bp and 1 611 bp long, encoding 758 and 536 amino acids, respectively. The kat B gene was cloned into pKK2233 expressing vector and transformed into the E. coli UM2, a catalasedeficient mutant. Staining of nondenatured PAGE for catalase activity demonstrated that kat B gene inserted into pKK2233 encoded a catalase that comigrated with the CatB found in D. radiodurans cell lysates. It was also observed that expression of kat B gene in E. coli UM2 could restore the resistance to H2O2 at low concentration. Fig 4, Ref 10

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Last Update: 2002-10-31