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Effects of Rare Earth Elements on Isoflavone Biosynthesis and Redox Status of Maackia amurensis Cells in Suspension Cultures(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2008 No.03
Page:
362-365
Research Field:
Reviews
Publishing date:

Info

Title:
Effects of Rare Earth Elements on Isoflavone Biosynthesis and Redox Status of Maackia amurensis Cells in Suspension Cultures
Author(s):
LUO Jianping et al.
(1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) (2Institute for Agricultural Application of Atomic Energy, Anhui Academy of Agricultural Sciences, Hefei 230031,China)
Keywords:
Keywords rare earth element cerium lanthanun isoflavone Maackia amurensis redox status
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Abstract:
Abstract To interpret the effects and mechamism of rare earth elements on isoflavone biosynthesis, the changes in isoflavone concentration, phenylalanine-ammonia lyase activity, and GSH and GSSG concentrations in the suspension cultures of Maackia amurensis were studied after Ce(NO3)3 and La(NO3)3 were added into the liquid medium. The rusults showed that the optimum concentrations of Ce(NO3)3 and La(NO3)3 for isoflavone biosynthesis were 20 mmol L-1 and 10 mmol L-1, respectively. During the culture period, the change in isoflavone biosynthesis caused by the elicitation of Ce(NO3)3 or La(NO3)3 was consistent with the activity of PAL. The highest accumulation of isoflavone genistein occurred on day 4 for the elicitation of Ce(NO3)3 and day 3 for La(NO3)3, and was 271.42 μg g-1 (DW) and 279.32 μg g-1 (DW), respectively. The analysis of cellular redox status showed that the treatment of Ce(NO3)3 and La(NO3)3 changed the levels of GSH and GSSG in the cultured cells. The ratio of GSH/GSSG reached a peak value on day 3 for Ce(NO3)3 and day 2 for La(NO3)3, and it was enhanced by 0.46 and 0.47 times as compared to the control. The obtained results suggested an increase in isoflavone biosynthesis by the rare earth elements could be related to the change in cell redox status. Fig 3, Ref 16

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Last Update: 2008-07-01