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Comparative Genomic Analysis of the GRF Transcription Factors and the Heterosis Effect in Camellia sinensis(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2022 04
Page:
1-10
Research Field:
Articles
Publishing date:

Info

Title:
Comparative Genomic Analysis of the GRF Transcription Factors and the Heterosis Effect in Camellia sinensis
Author(s):
HOU Binghao1 WANG Pengjie12 GUO Yongchun1 WANG Li3 GU Mengya1 ZHANG Xingtan2* & YE Naixing1*
1 College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science at Universities in Fujian , Fuzhou 350002, China
2 Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences , Shenzhen 518120, China
3 College of Tea and Food Science, Wuyi University, Wuyishan 353400 , China
Keywords:
Camellia sinensis Tie-guanyin Huangdan GRF transcription factor s comparative genome heterosis
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2021.06048
DocumentCode:

Abstract:
Growth regulating factor ( GRF) is a transcription factor family that plays an important role in regulating plant growth and development. In this study, bioinformatics analysis were used to analyze the GRF transcription factors in tea plant genome of ‘Tie- guanyin’, ‘Huangdan’ and ‘Shuchazao’. Respectively, 15, 16 and 14 CsGRF transcription factors were identified with complete characteristic domains of QLQ and WRC. Based on phylogenetic relationships, the CsGRF genes were divided into 6 groups and each group shared the similar gene structure and conserved protein motifs. A large number of cis-acting elements related to light response, hormone response and stress response were revealed from upstream promoter region of the CsGRF transcription factors. The transcriptome data of different tissues showed that CsGRF genes were strongly expressed in tender shoots, while the expression levels in mature leaves were low or not . Fluorescence quantitative result showed that the expression levels of CsGRF genes in ‘Tie- guanyin’ were significantly lower than ‘Huangdan’. The expression levels of CsGRF genes varied among different artificial F 1 hybrid progenies. The expression levels of CsGRF genes in ‘ Jinguanyin’ were generally higher than the female parent ‘Tie-guanyin’, but not significant. The expression levels of CsGRF3, CsGRF7, CsGRF10 and CsGRF12 in ‘ Jinguanyin’ were significantly higher than ‘Tie-guanyin’; CsGRF2, CsGRF5, CsGRF6 and CsGRF14 had ultra-high parent expression and CsGRF2 reached significant level. The expression levels of CsGRF3 and CsGRF7 in ‘ Jinmudan’ were significantly higher than ‘Tie-guanyin’. Except CsGRF6, the expression levels of CsGRF genes in ‘ Zimeigui’ were higher than ‘Tie-guanyin’; CsGRF1, CsGRF2, CsGRF4, CsGRF5, CsGRF9 and CsGRF14 had ultra-high parent expression and reached significant level except CsGRF9. It was speculated that the preferential expression of some CsGRF genes in hybrid progenies might be related to the formation of heterosis of tea growth vigor.

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Last Update: 2021-09-08