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Enrichment and phylogenetic analysis of syntrophic propionate-degrading consortia from anaerobic digestion of vinasse(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2020 04
Page:
1-8
Research Field:
Articles
Publishing date:

Info

Title:
Enrichment and phylogenetic analysis of syntrophic propionate-degrading consortia from anaerobic digestion of vinasse
Author(s):
XIE Tongtong WU Kaixuan CHI Mingmei SUN Xiaojiao ZHANG Peiyu YU Deshuang & QIU Yanling**
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China
Keywords:
vinasse anaerobic propionate degradation syntrophy Syntrophobacter Smithella
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.11057
DocumentCode:

Abstract:
To investigate the propionate-degrading community of vinasse biogas plant, primary enrichments were made with propionate as sole carbon source with two stages of the vinasse biogas slurry as inoculum. Two stable mesophilic methanogenic propionate-degrading enrichments JO-P and JN-P were obtained after one year of successive transfers. Propionate was converted to methane and acetate within 1.5-2 months. The bacterial and archaeal community structures were investigated with a 16S rRNA gene-based high-throughput sequencing technology. The results showed that the dominant propionate-degrading bacteria in the two enrichments were different. In culture JO-P, the predominant phylotypes were related to three types of cultured propionate-degrading species Smithella propionica (99% similarity in 16S rRNA gene sequence), Syntrophobacter wolinii (95% similarity) and Desulfobulbus propionicus (100% similarity), which accounted for 37.61%, 10.40% and 1.34% of the total number of bacterial community, respectively. In JN-P culture, phylotype related Syntrophobacter wolinii (95% similarity) accounted for 24.9%, which was potential propionate-oxidizing bacterium. The archaeal community in two cultures was also different. In JO-P culture, hydrogenotrophic methanogen Methanoculleus receptaculi (100% similarity) and acetotrophic methanogen Methanosaeta concilii (100% similarity) accounted for 95.09% and 4.85% of the total archaeal community, respectively. Hydrogenotrophic methanogens Methanoculleus receptaculi (100% similarity) was dominant in JN-P culture, which accounted for 99.98%. This study revealed that Smithella propionica, Desulfobulbus propionicus, and Syntrophobacter wolinii play important roles in propionate degradation of vinasse biogas plant.

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Last Update: 2020-01-14