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Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour.(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2020 04
Page:
1-14
Research Field:
Articles
Publishing date:

Info

Title:
Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour.
Author(s):
ZHU yongjing ZHANG shuting LU baoshun LIU Pudong LI xiaofei LAI Zhongxiong & LIN yuling
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Keywords:
Dimocarpus longan L cellulose synthase bioinformatics analysis expression analysis
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.10009
DocumentCode:

Abstract:
To clarify the biological function of the cellulose synthase (DlCESA) gene family in longan (Dimocarpus longan Lour.), the gene structure, protein characteristics, conserved motifs, cis-acting element of promoter, phylogenetic tree, and the FPKM values of DlCESA gene family were analyzed by the bioinformatics methods, and qRT-PCR was further used to detect the expression patterns of DlCESA. The results showed the DlCESA gene family contained nine members and their introns ranged from eight to thirteen. Physicochemical properties among different DlCESA proteins were similar and fifteen conserved motifs were found; Phylogenetic analysis showed DlCESA were clustered into six groups; cis-acting elements of promoter sequences analysis showed DlCESA contained a lot of plant hormone cis-acting elements and anaerobic elements; FPKM values showed that DlCESA genes high express ed in non-embryogenic cultures (NEC), and down-regulated in the process of early somatic embryogenesis, and DlCESA genes were expressed in different tissues except DlCESA7-2. qRT-PCR analysis showed that four DlCESA gene s showed higher expression level in the NEC stage,; ABA and MeJA down-regulated the expression of DlCESA genes. This study showed that DlCESA genes may be involved in the reproductive growth , the somatic embryogenesis of longan, and respond to abiotic stresses

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Last Update: 2020-01-15