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The variation of gut microbio ta, biochemical character of serum and serum metabolites before and after cub giant panda weaning*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2020 03
Page:
1-14
Research Field:
Articles
Publishing date:

Info

Title:
The variation of gut microbio ta, biochemical character of serum and serum metabolites before and after cub giant panda weaning*
Author(s):
Jin Lei1 De Qing 2 Li Caiwu3 Deng Wenwen3 Yang Shengzhi1 Zhou Yu3 Zeng Wen3 Feng Li3 Zhang Hemin3 He Yongguo 2** & Zou Likou1**
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China
2 Wolong special administrative region, Wolong 6 23004, China
3 China Conservation and Research Center for the Giant Panda, Dujiangyan 6 1130, China
Keywords:
cub giant panda weaning gut microbiome biochemical character of serum serum metabolites
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.07049
DocumentCode:

Abstract:
To investigate the variation of gut microbiomta, biochemical character of serum and serum metabolites in cub giant panda before and after weaning milk were analyzed by using high-throughput sequencing technology, biochemical?detection and liquid?chromatography-tandem?mass?spectrometry. We collected 24 fecal and 24 serum samples from 3 cub giant pandas before and after weaning milk. The results showed that the richness of cub giant panda’ gut microbiome decreased after weaning . For bacterial communities, Firmicutes was the most dominant phylum before weaning, while Proteobacteria was the most dominant phylum after weaning. Escherichia-Shigella , Streptococcus, Clostridium, Enterococcus and Lactobacillus significantly decreased after weaning ( LDA > 4), while Pseudomonas , Leuconostoc and Sphingobacterium increased (LDA > 4). For fungal communities, Ascomycota and Basidiomycota were the most dominant phyla before weaning, while Basidiomycota and Zygomycota were the most dominant phyla after weaning. Microidium significantly decreased after weaning (P < 0.05), while Cystofilobasidium, Guehomyces and Cryptococcus increased significantly. Besides, CK and LDH significantly decreased after weaning, while TB significantly increased (P < 0.05). Meanwhile, 54 and 46 metabolites significantly varied during weaning both under the normal and negative model, such as heme and allantoic acid etc. Interestingly, CK and LDH had significantly association with these metabolites (P < 0.05). Therefore, our results indicated that the microbes involved in cellulose or hemicellulose digest had high er abundance after weaning and it provided?evidenceto support giant panda utilizing bamboo via gut microbiome . Meanwhile, CK, LDH and TB may be used as markers for the identification of health after weaning, which could keep the healthof cub giant panda during weaning.

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Last Update: 2019-09-04