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Genome-wide evaluation of constitutive and regulated promoters in Saccharomyces cerevisiae in different carbon sources*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2020 03
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Genome-wide evaluation of constitutive and regulated promoters in Saccharomyces cerevisiae in different carbon sources*
MIAO Pu1 Gou Min23 CHEN Dong 1 TANG Yueqin23**
1 Sinopec Shanghai Engineering Co. Ltd., Shanghai 200120, China
2 Environmental Biotechnology Research Center, College of Architecture and Environment, Sichuan University, Chengdu 610065, China
3 Sichuan Environmental Protection Key Laboratory of Organic Waste Resource Utilization, Chengdu 610065, China
Promoter Saccharomyces cerevisiae Transcriptome Xylose utilization

Xylose is the second most abundant sugar in lignocellulosic biomass but not a natural carbon source for Saccharomyces cerevisiae. To increase the resource utilization efficiency and reduce the cost of bioethanol production, it is crucial to construct strains able to ferment xylose effectively. The efficient expression of exogenous genes and the regulation of native genes require the selection of promoters with appropriate expression strength. However, up to now, the researches on the strength , the stability, and the inducibility of promotors in S. cerevisiae when fermenting glucose, xylose or mixed sugar s is very limited. In this study, the promoter strength in an industrial xylose-utilizing strain in response to four carbon source conditions (glucose, xylose, and mixed sugar ) was evaluated genome-wide using transcriptome data. The results showed that the strength of most promoters changed in the different carbon sources other than the glucose fermentation state while 67 promotors kept the strength level under different sugar conditions. A series of promoter libraries for gene engineering were provided. PTEF1, PTEF2, PADH1, PCCW12, and some ribosomal protein promoters could serve as constitutive strong promoters for xylose-utilizing strain construction. Constitutive medium and weak promoters which could be provided for strain optimization were summarized. PYNR071C, PPUT1, and PDSF1 could be used as inducible promoters in the xylose fermentation state, driving high levels of gene expression only when necessary. The libraries of constitutive and inducible promoters with different strengths provided in this study is useful for gene engineering of xylose-utilizing S. cerevisiae strains




Last Update: 2019-09-04