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Cloning and expression analysis of the CsHAC1 gene in response to Huanglongbing ( HLB) infection in sweet orange*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2020 02
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Cloning and expression analysis of the CsHAC1 gene in response to Huanglongbing ( HLB) infection in sweet orange*
SHI Xiaobao1 WANG Xingqi1 CHE Jingru1 YANG Li 1 WU Junwei1 LUO Binbin1 WANG Mengge 1 ZHONG Yun 2** & CHENG Chunzhen 1**
1Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University , Fuzhou 350002, China
2Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences , Guangzhou 510640, China
Sweet orange Histone acetyltransferase Huanglongbing Interacting protein Epigenetic regulation

To reveal the response mechanism of Citrus sinensis histone acetyltransferase 1 gene ( CsHAC1) during the Citrus Huanglongbing (HLB) infection process , the gDNA and cDNA sequences of the gene were cloned by PCR and RT-PCR, followed by a series of bioinformatics analysis. Protein-protein interaction (PPI) prediction was also performed. Additionally, expression and correlation analysis of CsHAC1 and genes encoding proteins that predicted to interact with CsHAC1 were performed. The coding sequence (CDS) of this gene is 5307 bp in length. It was predicted to encode protein containing 1768 amino acids with no signal peptides nor transmembrane structure. Subcellular localization prediction result show that CsHAC1 is mainly localized in the nucleus. CsHAC1 contains multiple conserved domains such as ZnF_TAZ, PHD, HAT_KAT11, and ZnF_ZZ. The PPI prediction result revealed that CsHAC1 can interact with several proteins such as ZC3H19L, SUMOs and HAM1L and so on. Besides a large number of photoresponsive elements, some stress responsive (such as low temperature, defense and stress, anaerobic etc.) and phytohormone responsive (such as ABA, auxin, SA, etc.) elements were also identified in the CsHAC1 promoter by promoter cis-acting element prediction. Moreover, by analyzing the expression of CsHAC1 and its interacting protein-coding genes in sweet orange samples infected with HLB, we found that CsHAC1 expression was induced by HLB in leaf, and its expression was significant negatively correlated with that of HAM1L. CsHAC1, together with its interacting protein genes, contribute to the citrus response to HLB infection through epigenetic regulation.




Last Update: 2019-09-04