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Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2020 02
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Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis*
SUN Yue1 HU Boyang1 XU Xin1 LIU Zilu1 LI Xiaofeng1 ZHANG Guoqing1 2 **
1 College of Bioscience and Resource Environment, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs, Beijing 102206, China
2 College of Plant Science and Technology, Beijing University of Agriculture, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206, China
Abortiporus biennis culture conditions antioxidant activity laccase

Abortiporus biennis is a medicinal white-rot fungus with antioxidation and anti-tumor effects, which can degrade lignin and cause white decay of wood. In the present study, A. biennis strain WX-02 isolated from Wuxi, Jiangsu Province, was used as the material. Optimal culture conditions of mycelia, antioxidant activities, and Cu2+ induced laccase production of liquid fermentation were further investigated. Optimum culture conditions based on single factor experiments have revealed that the optimum carbon source for strain WX-02 mycelial growth was glucose, while the optimum nitrogen sources were beef extract and soybean powder. When the C/N ratio was 40/1, the mycelia demonstrates the greatest growth vigor. The optimum growth factors were determined to be corn flour and vitamin C, while the optimal pH and temperature were 7.0 and 32 ℃, respectively. With the culture conditions of a 10% (V/V) inoculation amount at 28 ℃ and 150 r/min for 72 h, the fermentation broth using PD medium manifested a total antioxidant activity of 2.76 ± 0.31 mmol/L (FeSO4) and 0.40 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 100.00 ± 0.00%, and SOD activity of 15.75 ± 0.25 U/mL. Whereas the fermentation broth using the optimum culture condition medium demonstrated a total antioxidant activity of 0.20 ± 0.03 mmol/L (FeSO4) and 0.16 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 59.06 ± 0.61%, and SOD activity of 15.65 ± 0.62 U/mL. Further study of Cu2+ on extracellular laccase production revealed that Cu2+ of 0.25 mmol/L can significantly enhanced the enzyme production with the highest activity of (1.82 ± 0.02) × 105 U/mL after the liquid fermentation at 28 ℃ at 150 r/min for 96 h, which was 1.72 times as much as the control group. The strain WX-02 demonstrated considerable high antioxidant and laccase activities, suggesting its potential applications in medicinal and environmental management.




Last Update: 2019-07-30