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Effects of nano-filled coatings with different antifouling properties on eukaryotic flora in the marine biofilm*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2020 02
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Effects of nano-filled coatings with different antifouling properties on eukaryotic flora in the marine biofilm*
GUO Changlu12 SHI Hongwei12 ZHANG Zhizhou12**
1. School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264200, China;
2. Marine Antifouling Engineering Technology Center of Shangdong Province, Weihai 264200, China
marine biofouling biofilm nano-filled coating eukaryotic community high-throughput sequencing Leptothecata

Objectives: Carbon nanotubes (CNTs) and polydimethylsiloxane (PDMS) -prepared low surface energy composite antifouling coatings have differential antifouling properties, but few studies have been undertaken on the structure and function of the eukaryotic flora on the coating surfaces with different antifouling capacities. Methods: Nano-filled coatings were prepared with carbon nanotubes and polydimethylsiloxane (CNTs-PDMS). Early biofilm samples were collected on marine hanging plates with good and poor antifouling scores. Biofilm metagenome DNA was extracted and analyzed by high-throughput sequencing of 18S rRNA V4 region to study the composition of eukaryotic community, especially the alpha and beta diversity indexes, the richness indexes, the abundance of OTUs (operational taxonomic unit), and the correlation between flora compositions and CNTs parameters/environmental factors. Results: In the time window of this 24-day study, the structural composition of eukaryotic flora on the coating surfaces changes greatly, but the diversity and richness index values of two types of coatings were similar. However, the changing trend of the diversity and richness indexes was increase-decrease-then increase again for coatings with high antifouling scores, while increase-decrease for coatings with low antifouling scores. Besides, the content of Leptothecata was several-fold to tens-fold lower in the coatings with better antifouling performance than those with poor antifouling performance. Conclusion: The compositional structure of eukaryotic flora on the surface of all CNTs-PDMS coatings changes significantly during the field test period; two types of antifouling coatings with differential antifouling performance had similar eukaryotic flora structures but the changing trends of the flora were apparently different. These preliminary observations are worth to be investigated further.




Last Update: 2019-07-30