|Table of Contents|

Screening and resistance analysis of one lactic acid bacteria cross-resistant to antibiotics and lead in vegetable waste*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2020 02
Page:
1-10
Research Field:
Articles
Publishing date:

Info

Title:
Screening and resistance analysis of one lactic acid bacteria cross-resistant to antibiotics and lead in vegetable waste*
Author(s):
HUANG Xiying ZHANG Juntao DENG Lisha & GU Yunfu**
Department of Applied Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
Keywords:
vegetable waste antibiotics heavy metals lactic acid bacteria cross resistance
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.05008
DocumentCode:

Abstract:
In order to explore the resistance ability of lactic acid bacteria (LAB) existed in the leaf surface of the waste vegetables under the synergistic selection of heavy metals and antibiotics, the environmental safety of LAB lived in the leaf surface of the Brassica chinensis var chinensis was evaluated. Pure culture method was used to isolate the culturable. LAB strains in the waste vegetable leaves, and then the resistance of the isolated LAB to both antibiotics (e.g. gentamicin, oxytetracycline, ciprofloxacin, chloramphenicol, penicillin and sulfamonomethoxine) and heavy metals (like lead ion Pb2+) was evaluated. The results showed that 50 LAB strains were successfully isolated from the leaves of the Brassica chinensis var chinensis. Among them, 15 strains showed resistance to the six tested antibiotics, and 10 strains were resistant to Pb2+. Among which Lab99 showed a minimum inhibitory concentration (MIC) value of 6000 mg/L to Pb2+, and its’ MIC values for gentamicin, penicillin and sulfamonomethoxine was at least 320 mg/L. In addition, strain Lab99 showed a cross-resistance to Pb2+ and gentamicin, sulfamonomethoxine, oxytetracycline and chloramphenicol. Further phylogenetic analysis showed strain Lab99 was closely related to Enterococcus faecium. Totally, the lactic acid bacteria strains in the leaves of Brassica chinensis var chinensis showed cross-resistance to Pb2+ and antibiotics, which may provide scientific clues for remediating the cross-contamination of heavy metals and antibiotics in environment

References

-

Memo

Memo:
-
Last Update: 2019-07-30