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Diversity of endophyte isolated from Ocimum basilicum L. and biological function(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2020 01
Page:
1-14
Research Field:
Articles
Publishing date:

Info

Title:
Diversity of endophyte isolated from Ocimum basilicum L. and biological function
Author(s):
HUANG Shufen1CHANG Huayu 1GU Licheng1SONG Xu1JIANG Baoru1TAN Zhiyuan1&PENG Guixiang2**
1 College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
Keywords:
Ocimum basilicum L. Endophytic bacteria Nitrogen fixation Biological function Promoting effect
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.04060
DocumentCode:

Abstract:
Ocimum basilicum L is an annual medicinal and edible plant of Labiatae. In order to investigate biodiversity and physiological function of endophytic bacteria, the plant Ocimum basilicum L. was chosen as experiment material. Endophytic bacteria were isolated and purified from the plant by LB, VM, DN and CCM mediums. Genetic diversity of the isolates were studied by insertion sequence-based PCR (IS-PCR) finger-printing. The 16S rRNA gene phylogenetic analysis and physiological and biochemical tests were used to identify the representative strains of all clusters. The biological functions of representative strains of various groups were further identified by traditional methods, and the strains with strong functions were selected for further rice seed germination and pot culture. As a result, 48 endophytic bacteria were isolated and purified from the Ocimum basilicum L, which were clustered into 13 groups with IS-PCR fingerprinting method, showing the diversity of endophytic bacteria in the Ocimum basilicum L. Biological function tests showed that the endophytic bacteria had abundant functions ,in which 9 strains had the function of nitrogen fixation, 8 solubility function, 10 potassium solubility, 12 auxin secretion, and 7 sideophore secretion. Two strains with excellent functions, H1041 and H4039, were screened out. Their fermentation could both significantly promote the germination of rice seeds and the growth of rice. This experiment showed that the endophytic strains isolated from Ocimum basilicum L. have abundant t diversity both in types and functions.

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Last Update: 2019-06-19