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Isolation and identification of a heterotrophic nitrifying strain of Agrobacterium sp . a nd its ammoxidation performance *(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2020 02
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Isolation and identification of a heterotrophic nitrifying strain of Agrobacterium sp . a nd its ammoxidation performance *
TAI Yong1 DENG Jingxuan1 REN Hongyan1 2 3** HUANG Zhengxin1 2 3 RUAN Wenquan1 2 3
1 School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China
2 Jiangsu Key Laboratory of Anaerobic Biotechnology , Wuxi 214122, China
3 Jiangsu Province Water Treatment Technology and Materials Collaborative Innovation Center , Suzhou, 215009
heterotrophic nitrifying bacterium Agrobacterium sp. ammonia oxidation ammonia monooxygenase (AMO)

The traditional wastewater treatment processes are complicat and nitrogen removal efficiency is low. In this paper, a heterotrophic nitrifying bacteria was enriched, isolated and screened from activated sludge of landfill leachate biochemical reaction tank which located in Taohuashan,Wuxi City, Jiangsu Province. The strain was identified by morphological observation and 16S rRNA sequence analysis. Meanwhile, the heterotrophic nitrification characteristics, ammonia oxidation gene function and the influencing factors of heterotrophic nitrification properties were studied. The results showed that the isolated heterotrophic nitrifying bacteria was Agrobacterium sp., and the NH4+-N removal rate could reach 99.77% after 32 h incubation, the TN removal rate was 96.99%, of which 59.62% TN was converted into cells growth as Internal nitrogen , 37.37% TN was converted into gaseous nitrogen, the accumulation of NO3--N and NO2--N was not detected. Moreover, the amplification of ammonia monooxygenase gene ( amoA) was successfully amplified. The ability of ammonia oxidation of BT1 strain was further proved. The single factor experiment results showed that the strain could remove more than 98.51% of NH4+-N under condition of 30 °C, C/N 10-15, pH 7.0-9.0 and 120-160 r/min, which could show strain’s good ammonia oxidation performance. The ammonia nitrogen tolerance of BT1 strain was better, and could grow well with 500 and 1000 mg/L ammonia nitrogen. The NH4+-N removal rate is exceeding 64.69%. The high-efficiency heterotrophic nitrification performance and excellent ammonia nitrogen tolerance could make the BT1 strain application in the actual wastewater treatment.




Last Update: 2019-09-04