|Table of Contents|

Colorimetric detection of genetically modified organisms (GMO s) based on loop-mediated isoth ermal amplification (LAMP)*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 06
Page:
1-9
Research Field:
T & M
Publishing date:

Info

Title:
Colorimetric detection of genetically modified organisms (GMO s) based on loop-mediated isoth ermal amplification (LAMP)*
Author(s):
WANG Jiayu1 2 DONG Juan1** & TANG Zhuo1**
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
loop-mediated isothermal amplification (LAMP) genetically modified (GM) gene-specific visualdetection on-site detection
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.04020
DocumentCode:

Abstract:
With the rapid development and application of genetically modified (GM) technology, its imperative to strictly supervise GM crops and their products . The rapid and accurate GMOs testing technology is an important support to complete this work. GM crops often contain different exogenous gene for various transgenic resistance. Therefore, these exogenous genes can be used as markers for screening and identifying the transgenic components based on nucleic acid detection technology. Here, the study was based on Loop-mediated isothermal amplification (LAMP) technology coupled with neutral red, an indicator dye, to establish colorimetric detection method for maize endogenous gene and common-used exogenous gene. Specific primers for endogenous gene IVR of maize and exogenous gene bar, pat, cry1 Ab were designed ba sed on LAMP, respectively. The results show that specific detection of IVR gene, bar gene in GM maize Bt176, pat gene in GM maize TC1507 and 59122 can be accomplished based on real-time LAMP fluorescence repor ting system. The here designed cry1Ab gene LAMP primers can specifically amplified GM maize Bt176, but invalid to GM maize Bt11 and MON810. The visual detection method was developed under the concentration of 5 mmol/L Tris-HCl and 100 μmol/L neutral red dye. The method was capable of detecting IVR gene and exogenous gene bar, pat, cry1Ab genes within 1 h . Based on our results, the visual results were consistent to r eal-time fluorescence LAMP results, indicating that our visual detection method is accurate and reliable . In conclusion, the here established visual LAMP method is capable of detecting GM maize. This method is rapid, simple, and shows the great potential of on-site detection.

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Last Update: 2019-04-30